Genetic vaccination with 'self' tyrosinase-related protein 2 causes melanoma eradication but not vitiligo
| Autor: | Bronte, V., Apolloni, E., Roberto RONCA, Zamboni, P., Overwijk, W. W., Surman, D. R., Restifo, N. P., Zanovello, P. |
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| Rok vydání: | 2000 |
| Předmět: |
Melanoma
Experimental Vitiligo Animals CD8-Positive T-Lymphocytes immunology Cancer Vaccines therapeutic use/toxicity Interferon Type I Intramolecular Oxidoreductases Killer Cells Natural Lymphocyte Culture Test Mixed Lymphocyte Depletion Melanoma Experimental immunology/therapy Mice Inbred BALB C Inbred C57BL Peptides chemistry/immunology Pregnancy Proteins Vaccines Synthetic etiology/immunology Article Mice Inbred BALB C Vaccines Synthetic Killer Cells Natural Mice Inbred C57BL Lymphocyte Culture Test Mixed |
| Zdroj: | Scopus-Elsevier |
| Popis: | “Self” melanocyte differentiation antigens are potential targets for specific melanoma immunotherapy. Vaccination against murine tyrosinase-related protein (TRP)-1/gp75 was shown recently to cause melanoma rejection, which was accompanied by autoimmune skin depigmentation (vitiligo). To further explore the linkage between immunotherapy and autoimmunity, we studied the response to vaccination with a related antigen, TRP-2. i.m. inoculation of plasmid DNA encoding murine trp-2 elicited antigen-specific CTLs that recognized the B16 mouse melanoma and protected the mice from challenge with tumor cells. Furthermore, mice bearing established s.c. B16 melanomas rejected the tumor upon vaccination with a recombinant vaccinia virus encoding trp-2. Depletion experiments showed that CD8+ lymphocytes and natural killer cells were crucial for the antitumor activity of the trp-2-encoding vaccines. Mice that rejected the tumor did not develop generalized vitiligo, indicating that protective immunity can be achieved in the absence of widespread autoimmune aggression. |
| Databáze: | OpenAIRE |
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