The two-component system CpxAR is Essential for Virulence in the phytopathogen bacteria Dickeya dadantii EC3937
| Autor: | Bontemps-Gallo, Sébastien, Madec, Edwige, Lacroix, Jean-Marie |
|---|---|
| Přispěvatelé: | Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
CpxAR
Virulence Pyridines D dadantii Gene Expression Regulation Bacterial Plants [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology plant pathogen Article stress Bacterial Proteins Enterobacteriaceae [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Phosphorylation Protein Kinases Plant Diseases |
| Zdroj: | Environmental Microbiology Environmental Microbiology, 2015, Pathogen Ecology, 17 (11), pp.4415-4428. ⟨10.1111/1462-2920.12874⟩ Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2015, Pathogen Ecology, 17 (11), pp.4415-4428. ⟨10.1111/1462-2920.12874⟩ |
| ISSN: | 1462-2912 1462-2920 |
| DOI: | 10.1111/1462-2920.12874⟩ |
| Popis: | International audience; The CpxAR two-component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two-component system was shown to be required for virulence of several zoo-pathogens but it has never been investigated in phyto-pathogens. In this paper, we investigate the role of the CpxAR two-component system in vitro and in vivo in Dickeya dadantii, an enterobacterial phytopathogen that causes soft-rot disease in a large variety of plant species. cpxA null mutant displays a constitutively phosphorylated CpxR phenotype as shown by direct analysis of phosphorylation of CpxR by a Phos-Tag retardation gel approach. Virulence in plants is completely abolished in cpxA or cpxR mutants of D. dadantii. In planta, CpxAR is only activated at an early stage of the infection process as shown by Phos-Tag and gene fusion analyses. To our knowledge, this is the first time that the timing of CpxAR phosphorelay activation has been investigated during the infection process by direct monitoring of response regulator phosphorylation. |
| Databáze: | OpenAIRE |
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