HIV-1 reverse transcriptase is capable of elongating derivatives of sequence specific noncomplementary oligodeoxynucleotides

Autor:	Igor Martiyanov, Od, Zakharova, Sottofattori E, Ga, Maksakova, Dv, Pyshnyi, Mazzei M, Balbi A, Litvak S, Tarrago-Litvak L, Ga, Nevinsky
Rok vydání:	1998
Předmět:	Binding Sites Oligodeoxyribonucleotides Poly T RNA Transfer Reverse Transcriptase Inhibitors Templates Genetic HIV Reverse Transcriptase DNA Primers
Zdroj:	Europe PubMed Central
ISSN:	1039-9712
Popis:	We have carried out a comparison of KM and Vmax values for various primers in the polymerization reaction catalyzed by the HIV-1 RT. The affinity of RT for complementary d(pT)6 containing two different 5'-end pyranone derivatives was 2-3 orders of magnitude higher (KM = 3-15 nM) than that of d(pT)6 (KM = 12.6 mM). Oligodeoxynucleotides (ODNs) noncomplementary to poly(A) template were not elongated by RT. However, derivatives of d(CAGGTG) containing the 5'-terminal chromone and coumarin related groups were efficient primers showing KM (30-300 nM) and Vmax (75-93%) values comparable with that for d(pT)10 (800 nM; 100%). The [d(CAGGTG)]ddT ODN derivatives were effective inhibitors of RT. The primer function of derivatives of noncomplementary ODNs appears to be due to the additional interactions of their 5'-terminal groups with the enzyme tRNA-binding site.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::64a71f56547610f6135c94c2da813a5d https://pubmed.ncbi.nlm.nih.gov/9739449 Zobrazit plný text záznamu Plný text