Influence of age on retinochoroidal healing processes after argon photocoagulation in C57bl/6j mice
| Autor: | Dot, C., Parier, V., Behar-Cohen, F., Benezra, D., Jonet, L., Goldenberg, B., Picard, E., Serge Camelo, Kozak, Y., May, F., Soubrane, G., Jeanny, J. C. |
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| Přispěvatelé: | Picard, Emilie, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Intercommunal de Créteil (CHIC), Hôpital Hôtel-Dieu [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assuta Medical Centre-Rishon [Israel], Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Hôpital d'Instruction des Armées Legouest, Service de Santé des Armées, Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
Aging Aging/pathology Animals Argon Chemokine CCL2/genetics Chemokine CCL2/metabolism Choroid/blood supply Choroid/pathology Choroidal Neovascularization/pathology Choroidal Neovascularization/surgery Gene Expression Regulation Glial Fibrillary Acidic Protein/genetics Glial Fibrillary Acidic Protein/metabolism Laser Coagulation Mice Mice Inbred C57BL RNA Messenger/genetics RNA Messenger/metabolism Retina/metabolism Retina/pathology Retinal Pigment Epithelium/pathology Retinal Pigment Epithelium/surgery Vascular Endothelial Growth Factor A/genetics Vascular Endothelial Growth Factor A/metabolism Wound Healing [SDV]Life Sciences [q-bio] Retinal Pigment Epithelium Retina MESH: Choroid MESH: Laser Coagulation MESH: Mice Inbred C57BL Glial Fibrillary Acidic Protein MESH: Glial Fibrillary Acidic Protein MESH: Aging MESH: Animals RNA Messenger MESH: Mice MESH: Chemokine CCL2 Chemokine CCL2 MESH: RNA Messenger Choroid MESH: Argon MESH: Choroidal Neovascularization MESH: Retina MESH: Vascular Endothelial Growth Factor A MESH: Gene Expression Regulation Choroidal Neovascularization MESH: Retinal Pigment Epithelium [SDV] Life Sciences [q-bio] MESH: Wound Healing Research Article |
| Zdroj: | Molecular Vision Molecular Vision, 2020, 15, pp.670-84 Molecular Vision, vol. 15, pp. 670-684 Scopus-Elsevier Molecular Vision, Molecular Vision, 2020, 15, pp.670-84 |
| ISSN: | 1090-0535 |
| Popis: | International audience; Purpose: To analyze the influence of age on retinochoroidal wound healing processes and on glial growth factor and cytokine mRNA expression profiles observed after argon laser photocoagulation.Methods: A cellular and morphometric study was performed that used 44 C57Bl/6J mice: 4-week-old mice (group I, n=8), 6-week-old mice (group II, n=8), 10-12-week-old mice (group III, n=14), and 1-year-old mice (group IV, n=14). All mice in these groups underwent a standard argon laser photocoagulation (50 microm, 400 mW, 0.05 s). Two separated lesions were created in each retina using a slit lamp delivery system. At 1, 3, 7, 14, 60 days, and 4 months after photocoagulation, mice from each of the four groups were sacrificed by carbon dioxide inhalation. Groups III and IV were also studied at 6, 7, and 8 months after photocoagulation. At each time point the enucleated eyes were either mounted in Tissue Tek (OCT), snap frozen and processed for immunohistochemistry or either flat mounted (left eyes of groups III and IV). To determine, by RT-PCR, the time course of glial fibrillary acidic protein (GFAP), vascular endothelial growth factor (VEGF), and monocyte chemotactic protein-1 (MCP-1) gene expression, we delivered ten laser burns (50 microm, 400 mW, 0.05 s) to each retina in 10-12-week-old mice (group III', n=10) and 1-year-old mice (group IV', n=10). Animals from Groups III' and IV' had the same age than those from Groups III and IV, but they received ten laser impacts in each eye and served for the molecular analysis. Mice from Groups III and IV received only two laser impacts per eye and served for the cellular and morphologic study. Retinal and choroidal tissues from these treated mice were collected at 16 h, and 1, 2, 3, and 7 days after photocoagulation. Two mice of each group did not receive photocoagulation and were used as controls.Results: In the cellular and morphologic study, the resultant retinal pigment epithelium interruption expanse was significantly different between the four groups. It was more concise and smaller in the oldest group IV (112.1 microm+/-11.4 versus 219.1 microm+/-12.2 in group III) p |
| Databáze: | OpenAIRE |
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