Impacts of Bone Marrow Stem Cells on Caspase-3 Levels after Spinal Cord Injury in Mice
| Autor: | Gashmardi, N., Hosseini, S. E., Mehrabani, D., Edalatmanesh, M. A., Zahra Khodabandeh |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Iranian Journal of Medical Sciences Scopus-Elsevier Iranian Journal of Medical Sciences, Vol 42, Iss 6, Pp 593-598 (2017) Europe PubMed Central |
| ISSN: | 1735-3688 0253-0716 |
| Popis: | Spinal cord injury (SCI) is a drastic disability that leads to spinal cord impairment. This study sought to determine the effects of bone marrow stem cells (BMSCs) on caspase-3 levels after acute SCI in mice. Forty-two mice were randomly divided into 3 groups: control (2 subcategories), subjected to no intervention; sham (3 subcategories), subjected to acute SCI; and experimental (2 subcategories), subjected to SCI and cell transplantation. In the experimental group, 2×105 BMSCs were injected intravenously 1 day after SCI. The mesenchymal property of the cells was assessed. The animals in the 3 groups were sacrificed 1, 21, and 35 days after the induction of injury and caspase-3 levels were evaluated using a caspase-3 assay kit. The obtained values were analyzed with ANOVA and Tukey tests using GraphPad and SPSS. Based on the assessments, the transplanted cells were spindle-shaped and were negative for the hematopoietic markers of CD34 and CD45 and positive for the expression of the mesenchymal marker of CD90 and osteogenic induction. The caspase-3 levels showed a significant increase in the sham and experimental groups in comparison to the control group. One day after SCI, the caspase-3 level was significantly higher in the sham group (1.157±0.117) than in the other groups (P |
| Databáze: | OpenAIRE |
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