Specific surface glycan decorations enable antimicrobial peptide resistance in plant-beneficial pseudomonads with insect-pathogenic properties
| Autor: | Kupferschmied, P., Chai, T., Flury, P., Blom, J., Smits, T.H., Maurhofer, M., Keel, C. |
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| Rok vydání: | 2016 |
| Předmět: |
Exopolysaccharides
Virulence fungi Animals Antimicrobial Cationic Peptides/pharmacology Bacterial Proteins/genetics Bacterial Proteins/metabolism Feeding Behavior Moths/microbiology Moths/physiology O Antigens/biosynthesis Plant Diseases/parasitology Plants/microbiology Plants/parasitology Pseudomonas/drug effects Pseudomonas/genetics Pseudomonas/metabolism Pseudomonas/pathogenicity Pseudomonas aeruginosa/drug effects Pseudomonas aeruginosa/genetics Pseudomonas aeruginosa/metabolism Pseudomonas aeruginosa/pathogenicity O Antigens O-antigen 570: Biologie Moths Plants Bacterial Proteins Pseudomonas Pseudomonas aeruginosa Antimicrobial Cationic Peptides Plant Diseases |
| Zdroj: | Environmental microbiology, vol. 18, no. 11, pp. 4265-4281 |
| ISSN: | 1462-2920 |
| Popis: | Some plant-beneficial pseudomonads can invade and kill pest insects in addition to their ability to protect plants from phytopathogens. We explored the genetic basis of O-polysaccharide (O-PS, O-antigen) biosynthesis in the representative insecticidal strains Pseudomonas protegens CHA0 and Pseudomonas chlororaphis PCL1391 and investigated its role in insect pathogenicity. Both strains produce two distinct forms of O-PS, but differ in the organization of their O-PS biosynthesis clusters. Biosynthesis of the dominant O-PS in both strains depends on a gene cluster similar to the O-specific antigen (OSA) cluster of Pseudomonas aeruginosa. In CHA0 and other P. protegens strains, the OSA cluster is extensively reduced and new clusters were acquired, resulting in high diversity of O-PS structures, possibly reflecting adaptation to different hosts. CHA0 mutants lacking the short OSA form of O-PS were significantly impaired in insect virulence in Galleria injection and Plutella feeding assays. CHA0, PCL1391, and other insecticidal pseudomonads exhibited high resistance to antimicrobial peptides, including cecropins that are central to insect immune defense. Resistance of both model strains depended on the dominant OSA-type O-PS. Our results suggest that O-antigen is essential for successful insect infection and illustrate, for the first time, its importance in resistance of Pseudomonas to antimicrobial peptides. |
| Databáze: | OpenAIRE |
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