Divergent effects of peripheral and global neuropeptide Y deletion
| Autor: | Wee, Natalie KY, Madunic, Ivana Vrhovac, Ivanisevic, Tonci, Sinder, Benjamin P, Kalajzic, Ivo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
EXPRESSION
Male Physiology Adipocyte Cortical Bone Mesenchymal Cells Neuropeptide Y Osteoblast Bone and Bones Mice LEPTIN APPETITE Bone Density mental disorders Cortical Bone Animals Neuropeptide Y IN-VIVO Adiposity Mice Knockout Science & Technology Adipocyte Mesenchymal Cells Osteoblast Neurosciences humanities RECEPTORS Mice Inbred C57BL MICE ADIPOSE-TISSUE Phenotype OBESITY CELLS Original Article Female Neurosciences & Neurology BONE Life Sciences & Biomedicine |
| Zdroj: | Journal of Musculoskeletal & Neuronal Interactions |
| ISSN: | 1108-7161 |
| Popis: | OBJECTIVES: Neuropeptide Y (NPY) is involved in the coordination of bone mass and adiposity. However, multiple NPY sources exist and their individual contribution to the skeleton and adiposity not known. The objectives of our study were to evaluate the effects of peripheral mesenchymal derived NPY to the skeleton and adiposity and to compare them to the global NPYKO model. METHODS: To study the role of mesenchymal-derived NPY, we crossed conditional NPY (NPYfl/fl) mice with Prx1cre to generate PrxNPYKO mice. The bone phenotype was assessed using micro-CT. The skeletal phenotype of PrxNPYKO mice was subsequently compared to global NPYKO model. We evaluated body weight, adiposity and functionally assessed the feeding response of NPY neurons to determine whether central NPY signaling was altered by Prx1cre. RESULTS: We identified the increase in cortical parameters in PrxNPYKO mice with no changes to cancellous bone. This was the opposite phenotype to global NPYKO mice generated from the same conditional allele. Male NPYKOmice have increased adiposity, while PrxNPYKO mice showed no difference, demonstrating that local mesenchymal-derived NPY does not influence adiposity. CONCLUSION: NPY mediates both positive and negative effects on bone mass via separate regulatory pathways. Deletion of mesenchymal-derived NPY had a positive effect on bone mass. ispartof: JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS vol:20 issue:4 pages:579-590 ispartof: location:Greece status: published |
| Databáze: | OpenAIRE |
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