Sensory receptor repertoire in cyprid antennules of the barnacle Balanus improvisus

Autor: Anna Abramova, Magnus Alm Rosenblad, Anders Blomberg, Tomas Axel Larsson
Rok vydání: 2018
Předmět:
Sensory Receptors
Physiology
Markov models
Social Sciences
Biochemistry
Ion Channels
Transient Receptor Potential Channels
Medicine and Health Sciences
Psychology
Hidden Markov models
Drosophila Melanogaster
Physics
Eukaryota
Genomics
Animal Models
Chemoreceptor Cells
Crustaceans
Insects
Electrophysiology
Experimental Organism Systems
Physical Sciences
Medicine
Sensory Perception
Drosophila
Transcriptome Analysis
Research Article
Signal Transduction
Arthropod Antennae
Arthropoda
Science
Biophysics
Neurophysiology
Research and Analysis Methods
Arthropod Proteins
Model Organisms
Genetics
Animals
Thoracica
Organisms
Biology and Life Sciences
Computational Biology
Proteins
Probability theory
Cell Biology
Ligand-Gated Ion Channels
Genome Analysis
Invertebrates
Animal Studies
Mathematics
Neuroscience
Barnacles
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 5, p e0216294 (2019)
ISSN: 1932-6203
Popis: Barnacle settlement involves sensing of a variety of exogenous cues. A pair of antennules is the main sensory organ that the cyprid larva uses to explore the surface. Antennules are equipped with a number of setae that have both chemo- and mechanosensing function. The current study explores the repertoire of sensory receptors in Balanus improvisus cyprid antennules with the goal to better understand sensory systems involved in the settling behavior of this species. We carried out transcriptome sequencing of dissected B. improvisus cyprid antennules. The generated transcriptome assembly was used to search for sensory receptors using HMM models. Among potential chemosensory genes, we identified the ionotropic receptors IR25a, IR8a and IR93a, and several divergent IR candidates to be expressed in the cyprid antennules. We found one gustatory-like receptor but no odorant receptors, chemosensory or odorant-binding proteins. Apart from chemosensory receptors, we also identified 13 potential mechanosensory genes represented by several transient receptor potential channels (TRP) subfamilies. Furthermore, we analyzed changes in expression profiles of IRs and TRPs during the B. improvisus settling process. Several of the sensory genes were differentially expressed during the course of larval settlement. This study gives expanded knowledge about the sensory systems present in barnacles, a taxonomic group for which only limited information about receptors is currently available. It furthermore serves as a starting point for more in depth studies of how sensory signaling affects settling behavior in barnacles with implications for preventing biofouling.
Databáze: OpenAIRE
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