[Cloning and determining the primary structure of fragments of the RNA-polymerase beta-subunit genes from representatives of Baikal picoplankton]

Autor:	Oa, Suturina, Julie SOUTOURINA, Ea, Semenova
Předmět:	Siberia Base Sequence Sequence Homology Amino Acid Molecular Sequence Data Phytoplankton Animals Humans Amino Acid Sequence DNA-Directed RNA Polymerases Cloning Molecular Polymerase Chain Reaction DNA Primers
Zdroj:	Europe PubMed Central
Popis:	Fragments of the RNA polymerase beta-subunit genes from representatives of picoplankton of Lake Baikal were cloned using polymerase chain reaction (PCR), and the resulting recombinant clones were sequenced. An analysis of 93 clones revealed 40 different sequences. A comparison of these nucleotide sequences and the corresponding amino acid sequences deduced with their homologues from databanks confirmed the high conservation of the RNA polymerase beta-subunit region involved in the formation of the enzyme active site.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::5c2b02c19358737847d1f5a6bd554795 http://europepmc.org/abstract/med/10079948 Zobrazit plný text záznamu