SSU1 Checkup, a rapid tool for detecting chromosomal rearrangements related to the SSU1 promoter in Saccharomyces cerevisiae: an ecological and technological study on wine yeast
| Autor: | Marullo, Philippe, Claisse, Olivier, Raymond Eder, Maria Laura, Börlin, Marine, Feghali, Nadine, Bernard, Margaux, Legras, Jean-Luc, Albertin, Warren, Rosa, Alberto Luis, Masneuf-Pomarede, Isabelle |
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| Přispěvatelé: | Unité de Recherche Oenologie [Villenave d'Ornon], Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BioLaffort [Floirac], Universidad Católica de Córdoba, Lebanese American University (LAU), University of Sassari, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
food and beverages
Translocation Multiplex PCR Microbiology MARKER ASSISTED SELECTION TRANSLOCATION purl.org/becyt/ford/1 [https] Domestication Marker assisted selection DOMESTICATION SULFITE RESISTANCE [SDE]Environmental Sciences purl.org/becyt/ford/1.6 [https] MULTIPLEX PCR Sulfite resistance Original Research |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, 2020, 11, pp.1-14. ⟨10.3389/fmicb.2020.01331⟩ Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.1-14. ⟨10.3389/fmicb.2020.01331⟩ CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2020.01331⟩ |
| Popis: | Chromosomal rearrangements (CR) such as translocations, duplications and inversions play a decisive role in the adaptation of microorganisms to specific environments. In enological Saccharomyces cerevisiae strains, CR involving the promoter region of the gene SSU1 lead to a higher sulfite tolerance by enhancing the SO2 efflux. To date, three different SSU1 associated CR events have been described, including translocations XV-t-XVI and VIII-t-XVI and inversion inv-XVI. In the present study, we developed a multiplex PCR method (SSU1 checkup) that allows a rapid characterization of these three chromosomal configurations in a single experiment. Nearly 600 S. cerevisiae strains collected from fermented grape juice were genotyped by microsatellite markers. We demonstrated that alleles of the SSU1 promoter are differently distributed according to the wine environment (cellar versus vineyard) and the nature of the grape juice. Moreover, rearranged SSU1 promoters are significantly enriched among commercial starters. In addition, the analysis of nearly isogenic strains collected in wine related environments demonstrated that the inheritance of these CR shapes the genetic diversity of clonal populations. Finally, the link between the nature of SSU1 promoter and the tolerance to sulfite was statistically validated in natural grape juice containing various SO2 concentrations. The SSU1 checkup is therefore a convenient new tool for addressing population genetics questions and for selecting yeast strains by using molecular markers. Fil: Marullo, Philippe. Universite de Bordeaux; Francia Fil: Claisse, Olivier. Universite de Bordeaux; Francia Fil: Raymond Eder, María Laura. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina Fil: Börlin, Marine. Universite de Bordeaux; Francia Fil: Feghali, Nadine. Lebanese University; Líbano Fil: Bernard, Margaux. Universite de Bordeaux; Francia Fil: Legras, Jean Luc. Université Montpellier II; Francia Fil: Albertin, Warren. Universite de Bordeaux; Francia Fil: Rosa, Alberto Luis. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina Fil: Masneuf Pomarede, Isabelle. Universite de Bordeaux; Francia |
| Databáze: | OpenAIRE |
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