Integrative genome-wide analysis reveals the role of WIP proteins in inhibition of growth and development

Autor: Roldan, Maria Victoria Gomez, Izhaq, Farhaj, Verdenaud, Marion, Eleblu, John, Haraghi, Aimen, Sommard, Vivien, Chambrier, Pierre, Latrasse, David, Jégu, Teddy, Benhamed, Moussa, Szécsi, Judit, Bendahmane, Mohammed, Boualem, Adnane, Bendahmane, Abdelhafid
Přispěvatelé: Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), ANR-17-CE20-0019,EPISEX,Vers la comprehension du controle epigenetique du determinisme du sexe chez les cucurbitaceae(2017), ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), European Project: 341076,EC:FP7:ERC,ERC-2013-ADG,SEXYPARTH(2014), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Institute of Plant Sciences Paris-Saclay
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Communications Biology, Vol 3, Iss 1, Pp 1-12 (2020)
Communications Biology
Communications Biology, 2020, 3 (1), pp.1-12. ⟨10.1038/s42003-020-0969-2⟩
Communications Biology, Nature Publishing Group, 2020, 3 (1), pp.1-12. ⟨10.1038/s42003-020-0969-2⟩
Communications Biology, Nature Publishing Group, 2020, 3 (1), ⟨10.1038/s42003-020-0969-2⟩
ISSN: 2399-3642
Popis: In cucurbits, CmWIP1 is a master gene controlling sex determination. To bring new insight in the function of CmWIP1, we investigated two Arabidopsis WIP transcription factors, AtWIP1/TT1 and AtWIP2/NTT. Using an inducible system we showed that WIPs are powerful inhibitor of growth and inducer of cell death. Using ChIP-seq and RNA-seq we revealed that most of the up-regulated genes bound by WIPs display a W-box motif, associated with stress signaling. In contrast, the down-regulated genes contain a GAGA motif, a known target of polycomb repressive complex. To validate the role of WIP proteins in inhibition of growth, we expressed AtWIP1/TT1 in carpel primordia and obtained male flowers, mimicking CmWIP1 function in melon. Using other promoters, we further demonstrated that WIPs can trigger growth arrest of both vegetative and reproductive organs. Our data supports an evolutionary conserved role of WIPs in recruiting gene networks controlling growth and adaptation to stress.
Maria V Gomez Roldan, Farhaj Izhaq et al. study the role of WIP transcription factors in Arabidopsis thaliana. Using an inducible system, they generate ChIP-seq and RNA-seq data that reveal how WIP proteins repress development and growth. Through ectopic expression of WIP in Arabidopsis carpels, they obtain male flower, mimicking sex determination in melon.
Databáze: OpenAIRE
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