Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes

Autor: Passoni, Gabriella, Langevin, Christelle, Palha, Nuno, Mounce, Bryan C., Briolat, Valérie, Affaticati, Pierre, De Job, Elodie, Joly, Jean-Stéphane, Vignuzzi, Marco, Saleh, Maria-Carla, Herbomel, Philippe, Boudinot, Pierre, Levraud, Jean-Pierre
Přispěvatelé: Unité de recherche Virologie et Immunologie Moléculaires (VIM), Institut National de la Recherche Agronomique (INRA), Macrophages et Développement de l’Immunité, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Populations virales et Pathogenèse, Institut des Neurosciences de Paris-Saclay (Neuro-PSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Virus et Interférence ARN, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], This work was supported by funding from the European Community's Seventh Framework Programme [FP7-PEOPLE-2011-ITN] under grant agreement no. PITN-GA-2011-289209 for the Marie Curie Initial Training Network FishForPharma, the Agence Nationale de la Recherche (Zebraflam grant ANR-10-MDI-009), a Domaine d'Interet Majeur Maladies Infectieuses (DIM-Malinf) grant from Région Ile-de-France, institutional support was from Institut National de la Recherche Agronomique (INRA), the Institut Pasteur and Centre National de la Recherche Scientifique. This work also benefited from the facilities and expertise of TEFOR – Investissement d'avenir – ANR-II-INBS-0014., ANR-10-MIDI-0009,ZebraFlam,Signaux et cellules de la réponse inflammatoire: suivi en temps réel chez un vertébré entier, le danio zébré(2010), European Project: 289209,EC:FP7:PEOPLE,FP7-PEOPLE-2011-ITN,FISHFORPHARMA(2012), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Populations virales et Pathogenèse - Viral Populations and Pathogenesis, Institut des Neurosciences Paris-Saclay (NeuroPSI), Virus et Interférence ARN - Viruses and RNA Interference, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Disease Models & Mechanisms
Disease Models & Mechanisms, Cambridge Company of Biologists, 2017, 10 (7), pp.847-857. ⟨10.1242/dmm.029231⟩
Disease Models and Mechanisms 7 (10), 847-857. (2017)
Disease Models & Mechanisms, 2017, 10 (7), pp.847-857. ⟨10.1242/dmm.029231⟩
Disease Models & Mechanisms, Vol 10, Iss 7, Pp 847-857 (2017)
ISSN: 1754-8403
1754-8411
Popis: Alphaviruses, such as chikungunya virus (CHIKV) and Sindbis virus (SINV), are vector-borne pathogens that cause acute illnesses in humans and are sometimes associated with neuropathies, especially in infants and elderly patients. Little is known about their mechanism of entry into the central nervous system (CNS), even for SINV, which has been used extensively as a model for viral encephalopathies. We previously established a CHIKV infection model in the optically transparent zebrafish larva; here we describe a new SINV infection model in this host. We imaged in vivo the onset and progression of the infection caused by intravenous SINV inoculation. Similar to that described for CHIKV, infection in the periphery was detected early and was transient, whereas CNS infection started at later time points and was persistent or progressive. We then tested the possible mechanisms of neuroinvasion by CHIKV and SINV. Neither virus relied on macrophage-mediated transport to access the CNS. CHIKV, but not SINV, always infects endothelial cells of the brain vasculature. By contrast, axonal transport was much more efficient with SINV than CHIKV, both from the periphery to the CNS and between neural tissues. Thus, the preferred mechanisms of neuroinvasion by these two related viruses are distinct, providing a powerful imaging-friendly system to compare mechanisms and prevention methods of encephalopathies.
Summary: Imaging of neuroinvasion in zebrafish shows that chikungunya virus first infects the blood-brain barrier, whereas Sindbis virus relies on axonal transport.
Databáze: OpenAIRE
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