Use of BODIPY‐Cholesterol (TF‐Chol) for Visualizing Lysosomal Cholesterol Accumulation
| Autor: | Hölttä-Vuori, M, Sezgin, E, Eggeling, C, Ikonen, E |
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| Přispěvatelé: | Medicum, Department of Anatomy, Lipid Trafficking Lab |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Boron Compounds
TF-cholesterol Niemann-Pick type C disease Niemann–Pick type C disease Cell Line cholesterol accumulation Niemann-Pick C1 Protein polycyclic compounds Humans lipid imaging Membrane Glycoproteins Esterification BODIPY-cholesterol Cell Membrane Intracellular Signaling Peptides and Proteins Biological Transport Intracellular Membranes Fibroblasts BODIPY‐cholesterol TF‐cholesterol Lysosomal Storage Diseases Cholesterol Microscopy Fluorescence PLASMA-MEMBRANE lipids (amino acids peptides and proteins) 3111 Biomedicine Traffic Interchange Carrier Proteins Lysosomes |
| Zdroj: | Traffic (Copenhagen, Denmark) |
| ISSN: | 1600-0854 1398-9219 |
| Popis: | Dipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol in terms of membrane partitioning. However, the suitability of TF-Chol for detecting lysosomal cholesterol deposition has recently been questioned. Here, we highlight the fact that the method of lipid delivery and the analysis of time-point both affect the membrane distribution and labeling pattern of TF-Chol, similarly as with radiolabeled cholesterol. Lysosomal sterol accumulation characteristic to a lysosomal storage disease is most readily detected when the probe is introduced via the physiological route, i.e. as a sterol fatty acid ester in low-density lipoprotein particles. When administered to cells from solvent, lysosomal sterol sequestration becomes evident after an overnight equilibration between membranes. Non |
| Databáze: | OpenAIRE |
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