Super-silent FRET Sensor Enables Live Cell Imaging and Flow Cytometric Stratification of Intracellular Serine Protease Activity in Neutrophils

Autor:	Craven, Thomas H., Avlonitis, Nicolaos, McDonald, Neil, Walton, Tashfeen, Scholefield, Emma, Akram, Ahsan R., Walsh, Timothy S., Haslett, Chris, Bradley, Mark, Dhaliwal, Kevin
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Microscopy Confocal Intravital Microscopy Neutrophils Point-of-Care Systems Primary Cell Culture lcsh:R lcsh:Medicine Flow Cytometry Article Fluorescence Healthy Volunteers Microscopy Fluorescence Molecular Probes Fluorescence Resonance Energy Transfer Humans lcsh:Q Serine Proteases lcsh:Science Cells Cultured
Zdroj:	Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) Scientific Reports Craven, T H, Avlonitis, N, McDonald, N, Walton, T, Scholefield, E, Akram, A R, Walsh, T S, Haslett, C, Bradley, M & Dhaliwal, K 2018, ' Super-silent FRET Sensor Enables Live Cell Imaging and Flow Cytometric Stratification of Intracellular Serine Protease Activity in Neutrophils ', Scientific Reports, vol. 8, no. 1, pp. 13490 . https://doi.org/10.1038/s41598-018-31391-9
ISSN:	2045-2322
DOI:	10.1038/s41598-018-31391-9
Popis:	Serine proteases are released by neutrophils to act primarily as antimicrobial proteins but excessive and unbalanced serine protease activity results in serious host tissue damage. Here the synthesis of a novel chemical sensor based on a multi-branched fluorescence quencher is reported. It is super-silent, exhibiting no fluorescence until de-quenched by the exemplar serine protease human neutrophil elastase, rapidly enters human neutrophils, and is inhibited by serine protease inhibitors. This sensor allows live imaging of intracellular serine protease activity within human neutrophils and demonstrates that the unique combination of a multivalent scaffold combined with a FRET peptide represents a novel and efficient strategy to generate super-silent sensors that permit the visualisation of intracellular proteases and may enable point of care whole blood profiling of neutrophils.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::59d730ebeb6c68a630a8bdbc07e456c4 http://link.springer.com/article/10.1038/s41598-018-31391-9 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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