Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish
| Autor: | Wehner, Daniel, Tsarouchas, Themistoklis M., Michael, Andria, Haase, Christa, Weidinger, Gilbert, Reimer, Michell M., Becker, Thomas, Becker, Catherina G. |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Nature Communications Nature Communications, Vol 8, Iss 1, Pp 1-17 (2017) Wehner, D, Tsarouchas, T M, Michael, A, Haase, C, Weidinger, G, Reimer, M M, Becker, T & Becker, C G 2017, ' Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish ', Nature Communications, vol. 8, 126 . https://doi.org/10.1038/s41467-017-00143-0 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-00143-0 |
| Popis: | The inhibitory extracellular matrix in a spinal lesion site is a major impediment to axonal regeneration in mammals. In contrast, the extracellular matrix in zebrafish allows substantial axon re-growth, leading to recovery of movement. However, little is known about regulation and composition of the growth-promoting extracellular matrix. Here we demonstrate that activity of the Wnt/β-catenin pathway in fibroblast-like cells in the lesion site is pivotal for axon re-growth and functional recovery. Wnt/β-catenin signaling induces expression of col12a1a/b and deposition of Collagen XII, which is necessary for axons to actively navigate the non-neural lesion site environment. Overexpression of col12a1a rescues the effects of Wnt/β-catenin pathway inhibition and is sufficient to accelerate regeneration. We demonstrate that in a vertebrate of high regenerative capacity, Wnt/β-catenin signaling controls the composition of the lesion site extracellular matrix and we identify Collagen XII as a promoter of axonal regeneration. These findings imply that the Wnt/β-catenin pathway and Collagen XII may be targets for extracellular matrix manipulations in non-regenerating species. Following spinal injury in zebrafish, non-neural cells establish an extracellular matrix to promote axon re-growth but how this is regulated is unclear. Here, the authors show that Wnt/β-catenin signaling in fibroblast-like cells at a lesion activates axon re-growth via deposition of Collagen XII. |
| Databáze: | OpenAIRE |
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