Nanomechanics of the endothelial glycocalyx in experimental sepsis
| Autor: | Wiesinger, V. (Véronique), Peters, W. (Wladimir), Chappell, D. (Daniel), Kentrup, D. (Dominik), Reuter, S.J. (Stefan), Pavenstädt, H. (Hermann), Oberleithner, H. (Hans), Kümpers, P. (Philipp) |
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| Přispěvatelé: | Universitäts- und Landesbibliothek Münster |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | PLoS ONE, Vol 8, Iss 11, p e80905 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The endothelial glycocalyx (eGC), a carbohydrate-rich layer lining the luminal side of the endothelium, regulates vascular adhesiveness and permeability. Although central to the pathophysiology of vascular barrier dysfunction in sepsis, glycocalyx damage has been generally understudied, in part because of the aberrancy of in vitro preparations and its degradation during tissue handling. The aim of this study was to analyze inflammation-induced damage of the eGC on living endothelial cells by atomic-force microscopy (AFM) nanoindentation technique. AFM revealed the existence of a mature eGC on the luminal endothelial surface of freshly isolated rodent aorta preparations ex vivo, as well as on cultured human pulmonary microvascular endothelial cells (HPMEC) in vitro. AFM detected a marked reduction in glycocalyx thickness (266 ± 12 vs. 137 ± 17 nm, P |
| Databáze: | OpenAIRE |
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