| Autor: |
Ikuko, Nakajima, Masaki, Endo, Takashi, Haji, Takaya, Moriguchi, Toshiya, Yamamoto |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Plant Biotechnol (Tokyo) |
| Popis: |
We established a method for embryogenic callus induction and highly efficient Agrobacterium-mediated genetic transformation of a table grape cultivar ‘Shine Muscat’ (Vitis labruscana). Embryogenic calli were induced using flower bud filaments from a dormant cane. Agrobacterium strain LBA4404 harboring the binary plasmid pBin19-sgfp, which contains the sgfp and nptII genes, was used to infect embryogenic calli. Infected calli were selected on 1/2 MS medium containing 5% maltose and 2% agar supplemented with 15 mg l(−1) kanamycin. Efficiency of transformation of regenerated plants reached nearly 100% as determined by PCR and Southern blot analyses. The developed method will open a new avenue for genome editing of ‘Shine Muscat’ and contribute to the advancement of grape breeding. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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