Screening of SIRT6 inhibitors and activators: A novel activator has an impact on breast cancer cells

Autor: Jonna Tenhunen, Tomáš Kučera, Marjo Huovinen, Jenni Küblbeck, Egils Bisenieks, Brigita Vigante, Zaiga Ogle, Gunars Duburs, Martin Doležal, Ruin Moaddel, Maija Lahtela-Kakkonen, Minna Rahnasto-Rilla
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Biomedicine & Pharmacotherapy, Vol 138, Iss, Pp 111452-(2021)
ISSN: 0753-3322
Popis: Sirtuin 6 (SIRT6), a member of sirtuin family (SIRT1-7), regulates a variety of cellular processes involved in aging, metabolism, and cancer. Dysregulation of SIRT6 is widely observed in different breast cancer subtypes; however, the role and function of SIRT6 in cancer development remain largely unexplored. The aim of this study was to identify novel compounds targeting SIRT6 which may provide a new approach in development of anti-cancer therapy for breast cancer. Virtual screening was utilized to discover potential compounds targeting SIRT6 for in vitro screening. In addition, novel 1,4-dihydropyridine derivatives were synthetized and further subjected for the screening. The impact of the compounds on the deacetylation activity of SIRT6 was determined with HPLC method. The anti-cancer activities were screened for a panel of breast cancer cells. A set of 1,4-dihydropyridine derivatives was identified as SIRT6 inhibitors. A SIRT6 activating compound, (2,4-dihydroxy-phenyl)–2-oxoethyl 2-(3-methyl-4-oxo-2-phenyl-4H-chromen-8-yl)acetate (later called as 4H-chromen), was discovered and it provided 30–40-fold maximal activation. 4H-chromen was proposed to bind similarly to quercetin and place to previously reported SIRT6 activator sites. 4H-chromen was investigated in various breast cancer cells, and it decreased cell proliferation in all cells as well as arrested cell cycle in triple negative cells. Overall, this study describes a highly potent SIRT6 activator and new inhibitors that represent a novel tool to study the mechanism of SIRT6 function.
Databáze: OpenAIRE
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