Estrogens Induce Rapid Cytoskeleton Re-Organization in Human Dermal Fibroblasts via the Non-Classical Receptor GPR30
| Autor: | Carnesecchi, Julie, Malbouyres, Marilyne, de Mets, Richard, Balland, Martial, Beauchef, Gallic, Vié, Katell, Chamot, Christophe, Lionnet, Claire, Ruggiero, Florence, Vanacker, Jean-Marc |
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| Přispěvatelé: | Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), SFR Biosciences, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Balland, Martial, École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Estradiol [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] MAP Kinase Signaling System [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] lcsh:R lcsh:Medicine Estrogens Dermis Fibroblasts Receptors G-Protein-Coupled Phosphatidylinositol 3-Kinases Receptors Estrogen Quinolines Estrogen Receptor beta Humans Female lcsh:Q Benzodioxoles lcsh:Science Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (3), pp.e0120672. ⟨10.1371/journal.pone.0120672⟩ PLoS ONE, 2015, 10 (3), pp.e0120672. ⟨10.1371/journal.pone.0120672⟩ PLoS ONE, Vol 10, Iss 3, p e0120672 (2015) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0120672⟩ |
| Popis: | International audience; The post-menopausal decrease in estrogen circulating levels results in rapid skin deterioration pointing out to a protective effect exerted by these hormones. The identity of the skincell type responding to estrogens is unclear as are the cellular and molecular processesthey elicit. Here, we reported that lack of estrogens induces rapid re-organization of thehuman dermal fibroblast cytoskeleton resulting in striking cell shape change. This morphological change was accompanied by a spatial re-organization of focal adhesion and a substantial reduction of their number as evidenced by vinculin and actin co-staining. Cellmorphology and cytoskeleton organization was fully restored upon 17β-estradiol (E2) addition. Treatment with specific ER antagonists and cycloheximide respectively showed thatthe E2 acts independently of the classical Estrogen Receptors and that cell shape changeis mediated by non-genomic mechanisms. E2 treatment resulted in a rapid and transientactivation of ERK1/2 but not Src or PI3K. We show that human fibroblasts express the nonclassical E2 receptor GPR30 and that its agonist G-1 phenocopies the effect of E2. Inhibiting GPR30 through treatment with the G-15 antagonist or specific shRNA impaired E2effects. Altogether, our data reveal a novel mechanism by which estrogens act on skin fibroblast by regulating cell shape through the non-classical G protein-coupled receptor GPR30and ERK1/2 activation. |
| Databáze: | OpenAIRE |
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