AtKC1 is a general modulator of Arabidopsis inward Shaker channel activity
| Autor: | Jeanguenin, Linda, Alcon, Carine, Duby, Geoffrey, Boeglin, Martin, Chérel, Isabelle, Gaillard, Isabelle, Zimmermann, Sabine, Sentenac, Hervé, Véry, Anne-Aliénor |
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| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Unité de Biochimie Physiologique, Université Catholique de Louvain (UCL)-Institut des Sciebce de la Vie, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Catholique de Louvain = Catholic University of Louvain (UCL)-Institut des Sciebce de la Vie |
| Rok vydání: | 2011 |
| Předmět: |
Potassium Channels
AtKC1 Recombinant Fusion Proteins Xenopus heteromerisation Arabidopsis Plant Roots Plant Cells [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Animals Biomass Protein Interaction Maps Potassium Channels Inwardly Rectifying Sequence Deletion subcellular localisation Arabidopsis Proteins Cell Membrane conditional targeting Plants Genetically Modified Plant Leaves Protein Subunits Potassium Channels Voltage-Gated expression pattern Mutagenesis Site-Directed Potassium Shaker Superfamily of Potassium Channels Protein Multimerization Shaker K+ channel |
| Zdroj: | Plant Journal Plant Journal, Wiley, 2011, 67 (4), pp.570-82. ⟨10.1111/j.1365-313X.2011.04617.x⟩ |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | International audience; A functional Shaker potassium channel requires assembly of four α-subunits encoded by a single gene or various genes from the Shaker family. In Arabidopsis thaliana, AtKC1, a Shaker α-subunit that is silent when expressed alone, has been shown to regulate the activity of AKT1 by forming heteromeric AtKC1-AKT1 channels. Here, we investigated whether AtKC1 is a general regulator of channel activity. Co-expression in Xenopus oocytes of a dominant negative (pore-mutated) AtKC1 subunit with the inward Shaker channel subunits KAT1, KAT2 or AKT2, or the outward subunits SKOR or GORK, revealed that the three inward subunits functionally interact with AtKC1 while the outward ones cannot. Localization experiments in plant protoplasts showed that KAT2 was able to re-locate AtKC1 fused to GFP from endomembranes to the plasma membrane, indicating that heteromeric AtKC1-KAT2 channels are efficiently targeted to the plasma membrane. Functional properties of heteromeric channels involving AtKC1 and KAT1, KAT2 or AKT2 were analysed by voltage clamp after co-expression of the respective subunits in Xenopus oocytes. AtKC1 behaved as a regulatory subunit within the heterotetrameric channel, reducing the macroscopic conductance and negatively shifting the channel activation potential. Expression studies showed that AtKC1 and its identified Shaker partners have overlapping expression patterns, supporting the hypothesis of a general regulation of inward channel activity by AtKC1 in planta. Lastly, AtKC1 disruption appeared to reduce plant biomass production, showing that AtKC1-mediated channel activity regulation is required for normal plant growth. |
| Databáze: | OpenAIRE |
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