Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines
| Autor: | Grenga, Lucia, Gallais, Fabrice, Pible, Olivier, Gaillard, Jean-Charles, Gouveia, Duarte, Batina, Hélène, Bazaline, Niza, Ruat, Sylvie, Culotta, Karen, Miotello, Guylaine, Debroas, Stéphanie, Roncato, Marie-Anne, Steinmetz, Gérard, Foissard, Charlotte, Desplan, Anne, Alpha-Bazin, Béatrice, Almunia, Christine, Gas, Fabienne, Bellanger, Laurent, Armengaud, Jean |
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| Přispěvatelé: | Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-17-CE18-0023,Phylopeptidomics,Identification rapide de bactéries pathogènes et résistances aux antibiotiques(2017) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Epidemiology viruses Immunology Microbiology Betacoronavirus viral protein detection Tandem Mass Spectrometry [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases vaccine Virology Chlorocebus aethiops Drug Discovery Animals host response Antigens Viral Vero Cells mass spectrometry SARS-CoV-2 Virion COVID-19 Viral Vaccines Articles General Medicine [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Infectious Diseases [SDV.IMM]Life Sciences [q-bio]/Immunology Parasitology infection kinetics Research Article |
| Zdroj: | Emerging Microbes & Infections Emerging microbes & infections Emerging microbes & infections, Earliest : Springer-Nature ; Latest : Taylor & Francis, 2020, 9 (1), pp.1712-1721. ⟨10.1080/22221751.2020.1791737⟩ Emerging microbes & infections, 2020, 9 (1), pp.1712-1721. ⟨10.1080/22221751.2020.1791737⟩ article-version (VoR) Version of Record |
| ISSN: | 2222-1751 |
| DOI: | 10.1080/22221751.2020.1791737 |
| Popis: | International audience; Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic. |
| Databáze: | OpenAIRE |
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