Transport and Catabolism of Carbohydrates by Neisseria meningitidis

Autor: Derkaoui, Meriem, Antunes, Ana, Nait Abdallah, Jamila, Poncet, Sandrine, Mazé, Alain, Ma Pham, Que Mai, Mokhtari, Abdelhamid, Deghmane, Ala-Eddine, Joyet, Philippe, Taha, Muhamed-Kheir, Deutscher, Josef
Přispěvatelé: MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Infections Bactériennes Invasives, Institut Pasteur [Paris], Department of biology [Guelma], Université du 8 Mai 1945 [Guelma, Algérie], Expression Génétique Microbienne (EGM (UMR_8261 / FRE_3630)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut de biologie physico-chimique (IBPC), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), This research was supported by a grant from the Agence Nationale de la Recherche (ANR-05-MIIM-031) to J.D. and M.-K.T., and the ‘Initiative d'Excellence' program of the French government (grant DYNAMO, ANR-11-LABX-0011). J.N.A. received a PhD fellowship from the INRA and A.M. from the Algerian government., ANR-05-MIIM-0031,Phospho Virul,Effets des protéine kinases à P-loop sur la virulence des bactéries pathogènes.(2005), Institut de biologie physico-chimique (IBPC), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie physico-chimique (IBPC (FR_550)), Institut Pasteur [Paris] (IP), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of Molecular Microbiology and Biotechnology
Journal of Molecular Microbiology and Biotechnology, Karger, 2016, 26 (5), pp.320-332. ⟨10.1159/000447093⟩
Journal of Molecular Microbiology and Biotechnology, 2016, 26 (5), pp.320-332. ⟨10.1159/000447093⟩
ISSN: 1464-1801
1660-2412
DOI: 10.1159/000447093⟩
Popis: International audience; We identified the genes encoding the proteins for the transport of glucose and maltose in Neisseria meningitidis strain 2C4-3. A mutant deleted for NMV_1892(glcP) no longer grew on glucose and deletion of NMV_0424(malY) prevented the utilization of maltose. We also purified and characterized glucokinase and α-phosphoglucomutase, which catalyze early catabolic steps of the two carbohydrates. N. meningitidis catabolizes the two carbohydrates either via the Entner-Doudoroff (ED) pathway or the pentose phosphate pathway, thereby forming glyceraldehyde-3-P and either pyruvate or fructose-6-P, respectively. We purified and characterized several key enzymes of the two pathways. The genes required for the transformation of glucose into gluconate-6-P and its further catabolism via the ED pathway are organized in two adjacent operons. N. meningitidis also contains genes encoding proteins which exhibit similarity to the gluconate transporter (NMV_2230) and gluconate kinase (NMV_2231) of Enterobacteriaceae and Firmicutes. However, gluconate might not be the real substrate of NMV_2230 because N. meningitidis was not able to grow on gluconate as the sole carbon source. Surprisingly, deletion of NMV_2230 stimulated growth in minimal medium in the presence and absence of glucose and drastically slowed the clearance of N. meningitidis cells from transgenic mice after intraperitoneal challenge.
Databáze: OpenAIRE
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