Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos

Autor: Dimitri, Fabrèges, Nathalie, Daniel, Véronique, Duranthon, Nadine, Peyriéras
Přispěvatelé: BioEmergences, Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), École nationale vétérinaire d'Alfort (ENVA), RGB-Net [TD 1101] and Epiconcept [FA 1201] COST actions, Ministère de l’Enseignement Supérieur et de la Recherche, Fondation des Treilles (Young Researcher Prize), France BioImaging infrastructure [ANR-10- INBS-04], Morphoscope2 [ANR-11-EQPX-0029], Région Île-de-France [InterDIM ISC11], CRB-Anim [ANR-11-INBS-0003], Revive [ANR-10-LABX-73], Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), UMR INRA-ENVA 1198 (BDR)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Research Report
3D+time 2-photon imaging
Cell death
MESH: Cell Death
MESH: Cell Differentiation
Nuclear Transfer Techniques
Cloning
Organism
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Green Fluorescent Proteins
MESH: Asymmetric Cell Division
Embryonic Development
MESH: Rabbits
Cell Count
MESH: Imaging
Three-Dimensional
Imaging
Three-Dimensional
MESH: Green Fluorescent Proteins
MESH: Pregnancy
MESH: Computer Simulation
Pregnancy
MESH: Cell Proliferation
MESH: Cloning
Organism
Animals
MESH: Embryonic Development
Cell Lineage
Computer Simulation
MESH: Animals
Cell Proliferation
Somatic cell nuclear transfer
Digital specimens
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
MESH: Cell Count
Asymmetric Cell Division
Spatial cell segregation
MESH: Blastocyst Inner Cell Mass
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Cell Differentiation
MESH: Nuclear Transfer Techniques
MESH: Cell Lineage
MESH: Male
Asymmetrical divisions
Rabbit pre-implantation development
Microscopy
Fluorescence
Multiphoton
In silico experimentation
Blastocyst Inner Cell Mass
MESH: Microscopy
Fluorescence
Multiphoton
Female
Rabbits
MESH: Female
Zdroj: Development (Cambridge, England)
Development (Cambridge, England), Company of Biologists, 2018, 145 (8), pp.1-7. ⟨10.1242/dev.152041⟩
Development (Cambridge, England), Company of Biologists, 2018, 145 (8), ⟨10.1242/dev.152041⟩
ISSN: 1477-9129
0950-1991
DOI: 10.1242/dev.152041⟩
Popis: Mammalian embryo cloning by nuclear transfer has a low success rate. This is hypothesized to correlate with a high variability of early developmental steps that segregate outer cells, which are fated to extra-embryonic tissues, from inner cells, which give rise to the embryo proper. Exploring the cell lineage of wild-type embryos and clones, imaged in toto until hatching, highlights the respective contributions of cell proliferation, death and asymmetric divisions to phenotypic variability. Preferential cell death of inner cells in clones, probably pertaining to the epigenetic plasticity of the transferred nucleus, is identified as a major difference with effects on the proportion of inner cell. In wild type and clones, similar patterns of outer cell asymmetric divisions are shown to be essential to the robust proportion of inner cells observed in wild type. Asymmetric inner cell division, which is not described in mice, is identified as a regulator of the proportion of inner cells and likely gives rise to resilient clones.
Summary: A unique quantitative approach based on complete reconstruction of the cell lineage that unveils an unknown mechanism of size control in cell populations of rabbit blastocysts, wild types or clones.
Databáze: OpenAIRE
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