Plasma membrane Ca2+‐ATPase 1 is required for maintaining atrial Ca2+ homeostasis and electrophysiological stability in the mouse

Autor: Wang, Yanwen, Wilson, Claire, Cartwright, Elizabeth J., Lei, Ming
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: The Journal of Physiology
Wang, Y, Wilson, C, Cartwright, E & Lei, M 2017, ' Plasma membrane Ca2+ ATPase 1 is required for maintaining atrial Ca2+ homeostasis and electrophysiological stability in the mouse ', The Journal of Physiology . https://doi.org/10.1113/JP274110
ISSN: 1469-7793
0022-3751
Popis: Key points The role of plasma membrane Ca2+‐ATPase 1 (PMCA1) in Ca2+ homeostasis and electrical stability in atrial tissue has been investigated at both organ and cellular levels in mice with cardiomyocyte‐specific deletion of PMCA1 (PMCA1cko)The PMCA1cko hearts became more susceptible to atrial arrhythmic stress conditions than PMCA1loxP/loxP hearts.PMCA1 deficiency alters cellular Ca2+ homeostasis under both baseline and stress conditions.PMCA1 is required for maintaining cellular Ca2+ homeostasis and electrical stability in murine atria under stress conditions. Abstract To determine the role of plasma membrane Ca2+‐ATPase 1 (PMCA1) in maintaining Ca2+ homeostasis and electrical stability in the atrium under physiological and stress conditions, mice with a cardiomyocyte‐specific deletion of PMCA1 (PMCA1cko) and their control littermates (PMCA1loxP/loxP) were studied at the organ and cellular levels. At the organ level, the PMCA1cko hearts became more susceptible to atrial arrhythmias under rapid programmed electrical stimulation compared with the PMCA1loxP/loxP hearts, and such arrhythmic events became more severe under Ca2+ overload conditions. At the cellular level, the occurrence of irregular‐type action potentials of PMCA1cko atrial myocytes increased significantly under Ca2+ overload conditions and/or at higher frequency of stimulation. The decay of Na+/Ca2+ exchanger current that followed a stimulation protocol was significantly prolonged in PMCA1cko atrial myocytes under basal conditions, with Ca2+ overload leading to even greater prolongation. In conclusion, PMCA1 is required for maintaining Ca2+ homeostasis and electrical stability in the atrium. This is particularly critical during fast removal of Ca2+ from the cytosol, which is required under stress conditions.
Key points The role of plasma membrane Ca2+‐ATPase 1 (PMCA1) in Ca2+ homeostasis and electrical stability in atrial tissue has been investigated at both organ and cellular levels in mice with cardiomyocyte‐specific deletion of PMCA1 (PMCA1cko)The PMCA1cko hearts became more susceptible to atrial arrhythmic stress conditions than PMCA1loxP/loxP hearts.PMCA1 deficiency alters cellular Ca2+ homeostasis under both baseline and stress conditions.PMCA1 is required for maintaining cellular Ca2+ homeostasis and electrical stability in murine atria under stress conditions.
Databáze: OpenAIRE
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