Experimental hypercholesterolemia induces apoptosis in the aortic valve

Autor:	Rajamannan, N. M., Sangiorgi, G., Springett, M., Arnold, K., Mohacsi, T., Luigi Giusto Spagnoli, Edwards, W. D., Tajik, A. J., Schwartz, R. S.
Rok vydání:	2001
Předmět:	Male Microscopy Animal Hypercholesterolemia Heart Valve Diseases Settore MED/11 - Malattie dell'Apparato Cardiovascolare Apoptosis Settore MED/08 - Anatomia Patologica Electron Disease Models Animal Microscopy Electron Cholesterol Aortic Valve Disease Models Animals Rabbits
Zdroj:	Scopus-Elsevier Europe PubMed Central
ISSN:	0966-8519
Popis:	Aortic valve disease is presently the number one indication for valve replacement in the United States, yet its molecular mechanisms remain unknown. As apoptosis (programmed cell death) occurs in degenerative disease states, it was postulated that experimental hypercholesterolemia is associated with apoptosis in rabbit aortic valves.New Zealand White rabbits (n = 8) were fed a 1% cholesterol diet for 12 weeks; control rabbits (n = 8) were fed a normal diet. After sacrifice of the animals, the aortic valves were dissected. Apoptosis was identified in the valvular lesion by TdT-mediated dUTP-biotin nick end-labeling (TUNEL) technique, and confirmed with transmission electron microscopy. The number of apoptotic cells was measured by computed morphometry.Valves from hypercholesterolemic rabbits showed an increase in apoptosis. TUNEL staining was identified in the atherosclerotic layer of hypercholesterolemic valves (0.1% of cells), but not in the cells of controls (p0.0001).Apoptosis is increased in rabbit aortic valves during experimental hypercholesterolemia. If fatal cellular degeneration occurs in hypercholesterolemic valve disease, these data suggest that apoptosis may play a role in the mechanism of valvular disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::4e2309b8051580c026ef8b4bbbe4cb42 https://pubmed.ncbi.nlm.nih.gov/11380101 Zobrazit plný text záznamu