A Comparison of In Vitro Nucleosome Positioning Mapped with Chicken, Frog and a Variety of Yeast Core Histones

Autor: Allan, James, Fraser, Ross M., Owen-hughes, Tom, Docherty, Kevin, Singh, Vijender
Rok vydání: 2013
Předmět:
Zdroj: Journal of Molecular Biology
Allan, J, Fraser, R M, Owen-hughes, T, Docherty, K & Singh, V 2013, ' A comparison of in vitro nucleosome positioning mapped with chicken, frog and a variety of yeast core histones ', Journal of Molecular Biology, vol. 485, no. 22, pp. 4206-22 . https://doi.org/10.1016/j.jmb.2013.07.019
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2013.07.019
Popis: Using high-throughput sequencing, we have mapped sequence-directed nucleosome positioning in vitro on four plasmid DNAs containing DNA fragments derived from the genomes of sheep, drosophila, human and yeast. Chromatins were prepared by reconstitution using chicken, frog and yeast core histones. We also assembled yeast chromatin in which histone H3 was replaced by the centromere-specific histone variant, Cse4. The positions occupied by recombinant frog and native chicken histones were found to be very similar. In contrast, nucleosomes containing the canonical yeast octamer or, in particular, the Cse4 octamer were assembled at distinct populations of locations, a property that was more apparent on particular genomic DNA fragments. The factors that may contribute to this variation in nucleosome positioning and the implications of the behavior are discussed.
Graphical Abstract
Highlights • Chromatins were formed on plasmids containing fragments of sheep, drosophila, human and yeast genomic DNAs with four types of histone octamer. • Nucleosome positioning was measured for each reconstitute. • Although similar, the binding profiles obtained with the yeast histone octamer, including one containing Cse4, were distinct from those obtained with chick or frog histones. • The difference in nucleosome positioning was best seen on AT-rich DNA substrates.
Databáze: OpenAIRE
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