Blastocystis in the faeces of children from six distant countries : prevalence, quantity, subtypes and the relation to the gut bacteriome
| Autor: | Cinek, Ondrej, Polackova, Katerina, Odeh, Rasha, Alassaf, Abeer, Kramná, Lenka, Ibekwe, MaryAnn Ugochi, Majaliwa, Edna Siima, Ahmadov, Gunduz, Elmahi, Bashir Mukhtar Elwasila, Mekki, Hanan, Oikarinen, Sami, Lebl, Jan, Abdullah, Mohammed Ahmed |
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| Přispěvatelé: | Tampere University, BioMediTech |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Azerbaijan Asia Adolescent Nigeria Blastocystis Infections Infectious and parasitic diseases RC109-216 DNA Ribosomal Tanzania Sudan Feces Bacteriome Prevalence Humans Child Asymptomatic Infections Jordan Research Genetic Variation DNA Protozoan Gastrointestinal Microbiome Czechoslovakia Type 1 diabetes Blastocystis Africa Female 3111 Biomedicine |
| Zdroj: | Parasites & Vectors, Vol 14, Iss 1, Pp 1-16 (2021) Parasites & Vectors |
| Popis: | Background Blastocystis is a human gut symbiont of yet undefined clinical significance. In a set of faecal samples collected from asymptomatic children of six distant populations, we first assessed the community profiles of protist 18S rDNA and then characterized Blastocystis subtypes and tested Blastocystis association with the faecal bacteriome community. Methods Stool samples were collected from 244 children and young persons (mean age 11.3 years, interquartile range 8.1–13.7) of six countries (Azerbaijan 51 subjects, Czechia 52, Jordan 40, Nigeria 27, Sudan 59 and Tanzania 15). The subjects showed no symptoms of infection. Amplicon profiling of the 18S rDNA was used for verification that Blastocystis was the most frequent protist, whereas specific real-time PCR showed its prevalence and quantity, and massive parallel amplicon sequencing defined the Blastocystis subtypes. The relation between Blastocystis and the stool bacteriome community was characterized using 16S rDNA profiling. Results Blastocystis was detected by specific PCR in 36% (88/244) stool samples and was the most often observed faecal protist. Children from Czechia and Jordan had significantly lower prevalence than children from the remaining countries. The most frequent subtype was ST3 (49%, 40/81 sequenced samples), followed by ST1 (36%) and ST2 (25%). Co-infection with two different subtypes was noted in 12% samples. The faecal bacteriome had higher richness in Blastocystis-positive samples, and Blastocystis was associated with significantly different community composition regardless of the country (p |
| Databáze: | OpenAIRE |
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