Persistent fibrosis, hypertrophy and sarcomere disorganisation after endoscopy-guided heart resection in adult Xenopus

Autor: Marshall, Lindsey, Vivien, Céline, Girardot, Fabrice, Péricard, Louise, Demeneix, Barbara A., Coen, Laurent, Chai, Norin
Přispěvatelé: Evolution des régulations endocriniennes (ERE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Muséum national d'Histoire naturelle (MNHN)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Sarcomeres
Time Factors
Cardiac Ventricles
Xenopus
Muscle Tissue
lcsh:Medicine
Surgical and Invasive Medical Procedures
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Research and Analysis Methods
Amputation
Surgical
Amphibians
Xenopus laevis
Model Organisms
Myofibrils
Animal Cells
Heart Regeneration
Medicine and Health Sciences
Morphogenesis
Regeneration
Animals
lcsh:Science
[SDV.BDD]Life Sciences [q-bio]/Development Biology
ComputingMilieux_MISCELLANEOUS
Muscle Cells
lcsh:R
Organisms
Biology and Life Sciences
Thoracic Surgery
Heart
Endoscopy
Animal Models
Cell Biology
Fibrosis
Up-Regulation
Biological Tissue
Experimental Organism Systems
Surgery
Computer-Assisted
Vertebrates
Cardiovascular Anatomy
Frogs
Female
lcsh:Q
Anatomy
Cellular Types
Organism Development
Biomarkers
Research Article
Developmental Biology
Zdroj: PLoS ONE, Vol 12, Iss 3, p e0173418 (2017)
PLoS ONE
PLoS ONE, Public Library of Science, 2017, 12 (3), pp.e0173418. ⟨10.1371/journal.pone.0173418⟩
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0173418⟩
Popis: Models of cardiac repair are needed to understand mechanisms underlying failure to regenerate in human cardiac tissue. Such studies are currently dominated by the use of zebrafish and mice. Remarkably, it is between these two evolutionary separated species that the adult cardiac regenerative capacity is thought to be lost, but causes of this difference remain largely unknown. Amphibians, evolutionary positioned between these two models, are of particular interest to help fill this lack of knowledge. We thus developed an endoscopy-based resection method to explore the consequences of cardiac injury in adult Xenopus laevis. This method allowed in situ live heart observation, standardised tissue amputation size and reproducibility. During the first week following amputation, gene expression of cell proliferation markers remained unchanged, whereas those relating to sarcomere organisation decreased and markers of inflammation, fibrosis and hypertrophy increased. One-month post-amputation, fibrosis and hypertrophy were evident at the injury site, persisting through 11 months. Moreover, cardiomyocyte sarcomere organisation deteriorated early following amputation, and was not completely recovered as far as 11 months later. We conclude that the adult Xenopus heart is unable to regenerate, displaying cellular and molecular marks of scarring. Our work suggests that, contrary to urodeles and teleosts, with the exception of medaka, adult anurans share a cardiac injury outcome similar to adult mammals. This observation is at odds with current hypotheses that link loss of cardiac regenerative capacity with acquisition of homeothermy.
Databáze: OpenAIRE
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