A Comparison of Human Neutrophils Acquired from Four Experimental Models of Inflammation
| Autor: | Maini, Alexander A., George, Marc J., Motwani, Madhur P., Day, Richard M., Gilroy, Derek W., O’Brien, Alastair J. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male Neutrophils Physiology Pathology and Laboratory Medicine Monocytes Neutrophil Activation White Blood Cells Spectrum Analysis Techniques Blister Animal Cells Medicine and Health Sciences Immune Response Routes of Administration Skin CD11b Antigen Hematology Flow Cytometry Intercellular Adhesion Molecule-1 Body Fluids Blood Cell Processes Spectrophotometry Cantharidin Medicine Cytophotometry Cellular Types Anatomy Research Article Adult Adolescent Immune Cells Science Immunology Dermatology Research and Analysis Methods Models Biological Young Adult Signs and Symptoms Phagocytosis Diagnostic Medicine Intradermal Injections Blisters Humans Inflammation Pharmacology Blood Cells Receptors IgG Biology and Life Sciences Cell Biology |
| Zdroj: | PLoS ONE, Vol 11, Iss 10, p e0165502 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Defects in neutrophil function have been implicated in a wide spectrum of clinical conditions. Several models are employed to study activated human neutrophils akin to those found at a site of inflammation. These include whole blood (WB) ex vivo stimulation with lipopolysaccharide (LPS) and in vivo techniques: cantharidin blister, skin windows and intra-dermal injection of UV-killed E.coli (UVKEc). Neutrophils obtained from these have never been compared. We compared the activation status of neutrophils from each technique in order to inform the optimal model for use in human studies. Healthy male volunteers were randomised to undergo one of the four techniques (n = 5/group). LPS: WB stimulated with 1ng/ml of LPS for 4 hours. Cantharidin: 12.5μl of 0.1% cantharidin elicited a single blister, aspirated at 24 hours. Skin windows: four 6mm mechanical-suction blisters created, de-roofed and an exudate-collection chamber placed over the windows for 4 hours before aspiration. UVKEc: 1.5 x 107 UVKEc injected intra-dermally. A single 10mm mechanical-suction blister formed and aspirated at 4 hours. Unstimulated WB used as the control. Flow cytometry was used to determine activation status using CD16, CD11b, CD54, CD62L and CD88. Functional status was assessed with a phagocytosis assay. The pattern of neutrophil activation was similar in all models. Neutrophil CD11b was elevated in all models, most markedly in UVKEc (p |
| Databáze: | OpenAIRE |
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