LC/MS/MS を用いた血液試料中の水酸化ポリ塩化ビフェニル(OH-PCBs)測定法の改良
| Autor: | Kazuhiro, Tobiishi, Shigeru, Suzuki, Takashi, Todaka, Hironori, Hirakawa, Tsuguhide, Hori, Jumboku, Kajiwara, Teruaki, Hirata, Takao, Iida, Hiroshi, Uchi, Masutaka, Furue |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | 福岡醫學雜誌. 104(4):128-135 |
| ISSN: | 0016-254X |
| Popis: | 水酸化ポリ塩化ビフェニル(OH-PCBs)は，人体内におけるPCBの主要代謝物である．LC/MS/MSを用いた血液試料中のOH-PCBs測定法の改良を行った．新しい粒径2µmのLCカラムを採用し，LC/MS/MS測定条件の最適化を行った．改良法では，5gの血液試料で従来法より感度が向上した．また，誘導体化のような試料の特別な処理も不要で，OH-PCB類，PCB類およびダイオキシン類の同一試料による同時定量も可能である． Hydroxylated polychlorinated biphenyls (OH-PCBs) are well known as metabolites of PCBs in the human body. We improved a measurement method for OH-PCBs in blood samples using LC/MS/MS. A new 2µm particle column was used, and the analytical conditions for the LC/MS/MS measurements were optimized. The improved method is more sensitive than the conventional method for 5g blood samples. And, this method is effective at determining the concentrations of OH-PCBs, PCBs and dioxins from the same sample without special treatment of the sample such as derivatization. |
| Databáze: | OpenAIRE |
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