Expression of human decay accelerating factor or membrane cofactor protein genes on mouse cells inhibits lysis by human complement
| Autor: | D J, White, T, Oglesby, M K, Liszewski, I, Tedja, D, Hourcade, M W, Wang, L, Wright, J, Wallwork, J P, Atkinson |
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| Rok vydání: | 1992 |
| Předmět: |
Cytotoxicity
Immunologic B-Lymphocytes Membrane Glycoproteins Base Sequence CD55 Antigens Cell Survival Molecular Sequence Data Transplantation Heterologous Membrane Proteins Mice Transgenic Blood Proteins Complement System Proteins Hybrid Cells Transfection Polymerase Chain Reaction Cell Line Membrane Cofactor Protein Mice Oligodeoxyribonucleotides Antigens CD Chromosomes Human Pair 1 Animals Humans Rabbits Plasmacytoma |
| Zdroj: | Transplant international : official journal of the European Society for Organ Transplantation. 5 |
| ISSN: | 0934-0874 |
| Popis: | Mouse cells expressing the human complement regulatory proteins decay accelerating factor (DAF) or membrane cofactor protein (MCP) were produced both by hybridoma technology and by transfection with the appropriate cDNAs. The expression of either or both of these products protected the mouse cell from lysis by human (though not rabbit) complement in the presence of naturally occurring human anti-mouse antibody. This effect could be abrogated by the addition of monoclonal antibody against DAF or MCP. These data suggested that the production of animals transgenic for human complement regulatory proteins should in principle be similarly protected from hyperacute xenograft rejection. |
| Databáze: | OpenAIRE |
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