Crosstalk Between Retinoic Acid and Sex-Related Genes Controls Germ Cell Fate and Gametogenesis in Medaka

Autor: Adolfi, Mateus C., Herpin, Amaury, Martinez-Bengochea, Anabel, Kneitz, Susanne, Regensburger, Martina, Grunwald, David J., Schartl, Manfred
Přispěvatelé: University of Würzburg = Universität Würzburg, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan University [Changsha] (HNU), Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Department of Human Genetics [Salt Lake City], University of Utah, The Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, AquaCRISPR (ANR-16-COFA-0004-01), TUNESAL (Research Project- HAVBRUK2, PN: 294971), AQUAEXCEL 3.0 European Project, and 111 Project (China, Grant no. D20007) projects, ANR-16-COFA-0004,AquaCrispr,Optimization of the CRISPR/Cas9 knock-in technology and application in salmon and trout(2016), European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015), Univ Wurzburg, INRA, Hunan Normal Univ, Universidade Estadual Paulista (Unesp), Univ Utah, Texas State Univ
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Frontiers in Cell and Developmental Biology
Frontiers in Cell and Developmental Biology, Frontiers media, 2021, 8, pp.1-12. ⟨10.3389/fcell.2020.613497⟩
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Frontiers in Cell and Developmental Biology, 2021, 8, pp.1-12. ⟨10.3389/fcell.2020.613497⟩
ISSN: 2296-634X
DOI: 10.3389/fcell.2020.613497⟩
Popis: Made available in DSpace on 2021-06-25T12:35:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-18 Deutsche Forschungsgemeinschaft AquaCRISPR TUNESAL (Research Project-HAVBRUK2) AQUAEXCEL 3.0 European Project 111 Project (China) Graduate School of Life Sciences (GSLS) Sex determination (SD) is a highly diverse and complex mechanism. In vertebrates, one of the first morphological differences between the sexes is the timing of initiation of the first meiosis, where its initiation occurs first in female and later in male. Thus, SD is intimately related to the responsiveness of the germ cells to undergo meiosis in a sex-specific manner. In some vertebrates, it has been reported that the timing for meiosis entry would be under control of retinoic acid (RA), through activation of Stra8. In this study, we used a fish model species for sex determination and lacking the stra8 gene, the Japanese medaka (Oryzias latipes), to investigate the connection between RA and the sex determination pathway. Exogenous RA treatments act as a stress factor inhibiting germ cell differentiation probably by activation of dmrt1a and amh. Disruption of the RA degrading enzyme gene cyp26a1 induced precocious meiosis and oogenesis in embryos/hatchlings of female and even some males. Transcriptome analyzes of cyp26a1-/-adult gonads revealed upregulation of genes related to germ cell differentiation and meiosis, in both ovaries and testes. Our findings show that germ cells respond to RA in a stra8 independent model species. The responsiveness to RA is conferred by sex-related genes, restricting its action to the sex differentiation period in both sexes. Univ Wurzburg, Dev Biochem, Bioctr, Wurzburg, Germany INRA, UR1037, Fish Physiol & Genom, Rennes, France Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Coll Life Sci, Changsha, Peoples R China Sao Paulo State Univ, Reprod & Mol Biol Grp, Dept Morphol, Inst Biosci Botucatu, Botucatu, SP, Brazil Univ Utah, Dept Human Genet, Salt Lake City, UT USA Texas State Univ, Xiphophorus Genet Stock Ctr, San Marcos, TX USA Sao Paulo State Univ, Reprod & Mol Biol Grp, Dept Morphol, Inst Biosci Botucatu, Botucatu, SP, Brazil Deutsche Forschungsgemeinschaft: SCHA 408/12-1 Deutsche Forschungsgemeinschaft: HE 7135/2-1 AquaCRISPR: ANR-16-COFA-0004-01 TUNESAL (Research Project-HAVBRUK2): PN: 294971 111 Project (China): D20007
Databáze: OpenAIRE
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