The EnvZ-OmpR Two-Component Signaling System Is Inactivated in a Mutant Devoid of Osmoregulated Periplasmic Glucans in Dickeya dadantii
| Autor: | Caby, Marine, Bontemps-Gallo, Sébastien, Gruau, Peggy, Delrue, Brigitte, Madec, Edwige, Lacroix, Jean-Marie |
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| Přispěvatelé: | Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Lille, This study was supported by the Centre National de la Recherche Scientifique (CNRS), the Université de Lille Sciences et Technologies, and the Ministère de l’Enseignement Supérieur et de la Recherche. MC and SB-G were funded by a doctoral fellowship of University of Lille. The funding bodies were not involved in the study design, data collection and analysis, decision to publish, or preparation of the manuscript., We thank Loic Brunet for the electronic microscopy. We are grateful to Dr. Ciaran Finn for the manuscript improvements., Université de Lille-Centre National de la Recherche Scientifique (CNRS), CNRS, Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF] |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 9 (2018) Frontiers in Microbiology, Frontiers Media, 2018, 9, pp.2459. ⟨10.3389/fmicb.2018.02459⟩ Frontiers in Microbiology, 2018, 9, pp.2459. ⟨10.3389/fmicb.2018.02459⟩ |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2018.02459⟩ |
| Popis: | International audience; Osmoregulated periplasmic glucans (OPGs) are general constituents of alpha-, beta-, and gamma-Proteobacteria. This polymer of glucose is required for full virulence of many pathogens including Dickeya dadantii (D. dadantii). The phytopathogenic enterobacterium D. dadantii causes soft-rot disease in a wide range of plants. An OPG-defective mutant is impaired in environment sensing. We previously demonstrated that (i) fluctuation of OPG concentration controlled the activation level of the RcsCDB system, and (ii) RcsCDB along with EnvZ/OmpR controlled the mechanism of OPG succinylation. These previous data lead us to explore whether OPGs are required for other two-component systems. In this study, we demonstrate that inactivation of the EnvZ/OmpR system in an OPG-defective mutant restores full synthesis of pectinase but only partial virulence. Unlike for the RcsCDB system, the EnvZ-OmpR system is not controlled by OPG concentration but requires OPGs for proper activation. |
| Databáze: | OpenAIRE |
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