ComB proteins expression levels determine Helicobacter pylori competence capacity
| Autor: | Corbinais, Christopher, Mathieu, Aurélie, Damke, Prashant P., Kortulewski, Thierry, Busso, Didier, Prado-Acosta, Mariano, Radicella, J. Pablo, Marsin, Stéphanie |
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| Přispěvatelé: | Laboratoire de Recherche sur l'Instabilité Génétique (LRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Stabilité génétique, Cellules Souches et Radiations (SCSR (U_967)), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Radiobiologie Cellulaire et Moléculaire (IRCM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), SERRE, Marie-Claude |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial FAAM DNA Repair Helicobacter pylori [SDV]Life Sciences [q-bio] Gene Expression Regulation Bacterial Dna Article Helicobacter Pylori Transformation [SDV] Life Sciences [q-bio] Ciencias Biológicas purl.org/becyt/ford/1 [https] Transformation Genetic Bacterial Proteins Biología Celular Microbiología Competence Mutation Operon purl.org/becyt/ford/1.6 [https] B3S CIENCIAS NATURALES Y EXACTAS DNA Damage |
| Zdroj: | Scientific Reports Scientific Reports, 2017, 7, pp.41495. ⟨10.1038/srep41495⟩ Scientific Reports, Nature Publishing Group, 2017, 7, pp.41495. ⟨10.1038/srep41495⟩ CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep41495⟩ |
| Popis: | Helicobacter pylori chronically colonises half of the world's human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and an efficient DNA recombination system, horizontal gene transfer through natural competence makes of H. pylori one of the most genetically diverse bacteria. We show here that transformation capacity is enhanced in strains defective for recN, extending previous work with other homologous recombination genes. However, inactivation of either mutY or polA has no effect on DNA transformation, suggesting that natural competence can be boosted in H. pylori by the persistence of DNA breaks but not by enhanced mutagenesis. The transformation efficiency of the different DNA repair impaired strains correlates with the number of transforming DNA foci formed on the cell surface and with the expression of comB8 and comB10 competence genes. Overexpression of the comB6-B10 operon is sufficient to increase the transformation capacity of a wild type strain, indicating that the ComB complex, present in the bacterial wall and essential for DNA uptake, can be a limiting factor for transformation efficiency. Fil: Corbinais, Christopher. Inserm; Francia. Institute of Molecular and Cellular Radiobiology; Francia. Université Paris Sud; Francia Fil: Mathieu, Aurélie. Institute of Molecular and Cellular Radiobiology; Francia Fil: Damke, Prashant P.. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia Fil: Kortulewski, Thierry. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia Fil: Busso, Didier. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia Fil: Prado Acosta, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institute of Molecular and Cellular Radiobiology; Francia Fil: Radicella, J. Pablo. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia Fil: Marsin, Stéphanie. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia |
| Databáze: | OpenAIRE |
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