Role of Dectin-2 for Host Defense against Systemic Infection with Candida glabrata
| Autor: | Daniela C, Ifrim, Judith M, Bain, Delyth M, Reid, Marije, Oosting, Ineke, Verschueren, Neil A R, Gow, J Han, van Krieken, Gordon D, Brown, Bart-Jan, Kullberg, Leo A B, Joosten, Jos W M, van der Meer, Frank, Koentgen, Lars P, Erwig, Jessica, Quintin, Mihai G, Netea |
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| Přispěvatelé: | Radboud University Medical Center [Nijmegen], University of Aberdeen, Ozgene |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Neutrophils
[SDV]Life Sciences [q-bio] Candida glabrata MESH: Neutrophils [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Mice Phagocytosis MESH: Mice Inbred C57BL Candida albicans Animals Lectins C-Type MESH: Animals MESH: Phagocytosis MESH: Candida glabrata MESH: Mice [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Host Response and Inflammation MESH: Cytokines Macrophages MESH: Candida albicans Candidiasis MESH: Reactive Oxygen Species MESH: Macrophages Th1 Cells MESH: Candidiasis Mice Inbred C57BL [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Th1 Cells Cytokines [SDV.IMM]Life Sciences [q-bio]/Immunology Female Reactive Oxygen Species MESH: Female MESH: Lectins C-Type |
| Zdroj: | Infection and Immunity Infection and Immunity, American Society for Microbiology, 2014, 82 (3), pp.1064-1073. ⟨10.1128/IAI.01189-13⟩ |
| ISSN: | 0019-9567 1098-5522 |
| DOI: | 10.1128/IAI.01189-13⟩ |
| Popis: | International audience; Although Candida glabrata is an important pathogenic Candida species, relatively little is known about its innate immune recognition. Here, we explore the potential role of Dectin-2 for host defense against C. glabrata. Dectin-2-deficient (Dectin-2(-/-)) mice were found to be more susceptible to C. glabrata infections, showing a defective fungal clearance in kidneys but not in the liver. The increased susceptibility to infection was accompanied by lower production of T helper 1 (Th1) and Th17-derived cytokines by splenocytes of Dectin-2(-/-) mice, while macrophage-derived cytokines were less affected. These defects were associated with a moderate yet significant decrease in phagocytosis of the fungus by the Dectin-2(-/-) macrophages and neutrophils. Neutrophils of Dectin-2(-/-) mice also displayed lower production of reactive oxygen species (ROS) upon challenge with opsonized C. glabrata or C. albicans. This study suggests that Dectin-2 is important in host defense against C. glabrata and provides new insights into the host defense mechanisms against this important fungal pathogen. |
| Databáze: | OpenAIRE |
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