Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: An in vitro and in vivo study

Autor: Imperatore, R, Carotenuto, G, Di Grazia MA, Ferrandino, I, Palomba, Letizia, Mariotti, R, Vitale, E, De Nicola, S, Longo, A, Cristino, L.
Přispěvatelé: Imperatore, R., Carotenuto, G., Di Grazia, Ma, Ferrandino, I., Palomba, L, Mariotti, R., Vitale, E., De Nicola, S., Longo, A., Cristino, L.
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Journal of biomedical materials research. Part A 103 (2015): 1436–1446. doi:10.1002/jbm.a.35289
info:cnr-pdr/source/autori:Imperatore R, Carotenuto G, Di Grazia MA, Ferrandino I, Palomba L, Mariotti R, Vitale E, De Nicola S, Longo A, Cristino L./titolo:Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: An in vitro and in vivo study/doi:10.1002%2Fjbm.a.35289/rivista:Journal of biomedical materials research. Part A/anno:2015/pagina_da:1436/pagina_a:1446/intervallo_pagine:1436–1446/volume:103
info:cnr-pdr/source/autori:Imperatore, Roberta; Carotenuto, Gianfranco; Di Grazia, Maria Antonietta; Ferrandino, Ida; Palomba, Letizia; Vitale, Emilia; Mariotti, Raffaella; De Nicola, Sergio; Longo, Angela; Cristino, Luigia/titolo:Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: An in vitro and in vivo study/doi:10.1002%2Fjbm.a.35289/rivista:Journal of biomedical materials research. Part A/anno:2015/pagina_da:1436/pagina_a:1446/intervallo_pagine:1436–1446/volume:103
Journal of biomedical materials research. Part A 103 (2015). doi:10.1002/jbm.a.35289
info:cnr-pdr/source/autori:1) Imperatore R, Carotenuto G, 1) Di Grazia MA, Ferrandino I, 1) Palomba L, Mariotti R, 2) Vitale E, De Nicola S, Longo A, 1) Cristino L/titolo:Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: an in vitro and in vivo study./doi:10.1002%2Fjbm.a.35289/rivista:Journal of biomedical materials research. Part A/anno:2015/pagina_da:/pagina_a:/intervallo_pagine:/volume:103
DOI: 10.1002/jbm.a.35289
Popis: Gold nanoparticles are increasingly being employed in innovative biological applications thanks to their advantages of material- and size-dependent physics and chemical interactions with the cellular systems. On the other hand, growing concern has emerged on the toxicity which would render gold-based nanoparticles harmful to cell cultures, animals, and humans. Emerging attention is focused on the interaction of gold nanoparticles with nervous system, especially regarding the ability to overcome the blood-brain barrier (BBB) which represents the major impediment to the delivery of therapeutics into the brain. We synthesized highly stable 2-mercapto-1-methylimidazole-stabilized gold-nanoparticles (AuNPs)-mmi to investigate their entry, accumulation, and toxicity in vitro (SH-SY5Y human neuroblastoma cells) and in vivo (brain of C57BL/6 mice) through optical and electron microscopy. After incubation in the cell culture medium at the lowest dose of 0.1 mg/mL the (AuNPs)-mmi nanoparticles were found compacted and recruited into endosome/lysosomes (1 h) before their fusion (2 h) and the onset of neuronal death by apoptosis (4 h) as proved by terminal-transferase-mediated dUTP nick end labeling assay and caspase-3 immunoreactivity. The ability of (AuNPs)-mmi to cross the BBB was assessed by injection in the caudal vein of C57BL/6 mice. Among different brain regions, the nanoparticles were found in the CaudatoPutamen area, mainly in the striatal neurons 4 h after injection. These neurons showed the typical hallmarks of apoptosis. Our findings provide, for the first time, the dynamic of 2-mercapto-1-methylimidazole nanogold uptake. The molecular mechanism which underlies the nanogold-driven apoptotic event is analyzed and discussed in order to take into account when designing nanomaterials to interface with biological structures. © 2014 Wiley Periodicals, Inc.
Databáze: OpenAIRE
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