Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran
| Autor: | Hanifian, H., Diba, K., Hazrati Tappeh, K., Mohammadzadeh, H., Rahim Mahmodlou |
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| Zdroj: | Europe PubMed Central Scopus-Elsevier Iranian Journal of Parasitology, Vol 8, Iss 3, Pp 376-381 (2013) Iranian Journal of Parasitology, Vol 8, Iss 3 (2013) Iranian Journal of Parasitology |
| Popis: | Background: The aim of this study was DNA extraction from protosco-lecses of Echinococcus granulosus and identification of these strains in West- Azerbaijan Province, north western Iran. Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different re-striction enzymes of Taq 1, Rsa 1 and Alu 1. Result: Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analy-sis also were the same for all isolates. PCR-RFLP patterns restriction en-zymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu1 bands were as follows: two approx-imately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples. Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is domi-nant genotype in this province. |
| Databáze: | OpenAIRE |
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