Maize endosperm secretes a novel antifungal protein into adjacent maternal tissue
| Autor: | Serna, A., Maitz, M., O'Connell, T., Santandrea, G., Thevissen, K., Tienens, K., Hueros, G., Faleri, C., Cai, G., Lottspeich, F., Thompson, R.D. |
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| Přispěvatelé: | UMR 0102 - Unité de Recherche Génétique et Ecophysiologie des Légumineuses, Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Antifungal Agents
DNA Complementary DNA Plant Molecular Sequence Data Genes Plant Zea mays [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics endosperm [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics Tissue Distribution Amino Acid Sequence ComputingMilieux_MISCELLANEOUS In Situ Hybridization Plant Proteins Sequence Homology Amino Acid fungi Fungi food and beverages Biological Transport protease transfer layer antifungal defensin transport Seeds Sequence Alignment Signal Transduction Transcription Factors |
| Zdroj: | The Plant Journal Plant Journal Plant Journal, Wiley, 2001, 25 (6), pp.687-698 |
| ISSN: | 0960-7412 1365-313X |
| Popis: | A series of endosperm transfer layer-specific transcripts has been identified in maize by differential screening of a cDNA library of transcripts at 10 days after pollination. Sequence comparisons revealed among this class of cDNAs a novel, small gene family of highly diverged sequences encoding basal layer antifungal proteins (BAPs). The bap genes mapped to two loci on chromosomes 4 and 10. So far, bap-homologous sequences have been detected only in maize, teosinte and sorghum, and are not present in grasses outside the Andropogoneae tribe. BAP2 is synthesized as a pre-proprotein, and is processed by successive removal of a signal peptide and a 29-residue prodomain. The proprotein can be detected exclusively in microsomal membrane-containing fractions of kernel extracts. Immunolocalization reveals BAP2 to be predominantly located in the placentochalazal cells of the pedicel, adjacent to the basal endosperm transfer layer (BETL) cells, although the BAP2 transcript is found only in the BETL cells. The biological roles of BAP2 propeptide and mature peptide have been investigated by heterologous expression of the proprotein in Escherichia coli, and by tests of its fungistatic activity and that of the fully processed form in vitro. The mature BAP2 peptide exhibits potent broad-range activity against a range of filamentous fungi, including several plant pathogens. |
| Databáze: | OpenAIRE |
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