Quantitative evaluation of healthy epidermis by means of multiphoton microscopy and fluorescence lifetime imaging microscopy
| Autor: | Benati, E., Bellini, V., Borsari, S., Dunsby, C., Ferrari, C., French, P., Guanti, M., Guardoli, D., Koenig, K., Pellacani, Giovanni, Ponti, Giovanni, Schianchi, S., Talbot, C., Seidenari, S. |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Aged 80 and over Male Aging skin morphology high-resolution imaging Reproducibility of Results Dermoscopy Middle Aged Sensitivity and Specificity fluorescence lifetime imaging Healthy Volunteers multiphoton microscopy epidermis keratinocyte Skin Aging Young Adult Microscopy Fluorescence Multiphoton Epidermal Cells Image Interpretation Computer-Assisted Humans Female skin ageing Aged |
| Zdroj: | Skin Research and Technology; Vol 17 Skin research and technology 17 (2011): 295–303. doi:10.1111/j.1600-0846.2011.00496.x info:cnr-pdr/source/autori:Benati, Elisa; Bellini, Valerio; Borsari, Stefania; Dunsby, Christopher; Ferrari, Chiara; French, Paul; Guanti, Mario; Guardoli, Davide; Koenig, Karsten; Pellacani, Giovanni; Ponti, Giovanni; Schianchi, Simona; Talbot, Clifford; Seidenari, Stefania/titolo:Quantitative evaluation of healthy epidermis by means of multiphoton microscopy and fluorescence lifetime imaging microscopy/doi:10.1111%2Fj.1600-0846.2011.00496.x/rivista:Skin research and technology/anno:2011/pagina_da:295/pagina_a:303/intervallo_pagine:295–303/volume:17 |
| ISSN: | 1600-0846 |
| DOI: | 10.1111/j.1600-0846.2011.00496.x |
| Popis: | Multiphoton microscopy (MPM) enables the assessment of unstained living biological tissue with submicron resolution, whereas fluorescence lifetime imaging microscopy (FLIM) generates image contrast between different states of tissue characterized by various fluorescence decay rates. The aim of this study was to compare the healthy skin of young individuals with that of older subjects, as well as to assess the skin at different body sites, by means of MPM and FLIM.Nineteen elderly patients were examined on the outer side of the forearm, whereas 30 young individuals were assessed on the dorsal and volar sides of the forearm and on the thigh.Cell and nucleus diameters, cell density and FLIM vary according to the epidermal cell depth and the skin site. In elderly subjects, epidermal cells show morphologic alterations in shape and size, with smaller cell and nucleus diameters; the number of basal cells is decreased, whereas the mean fluorescence lifetimes at both the upper and the lower layers increase.This study provides quantitative and qualitative data on normal epidermis at different skin sites at different ages and represents a reference for the clinician attempting to understand the effectiveness of MPM and FLIM in discriminating diseased states of the skin from normal ones. |
| Databáze: | OpenAIRE |
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