Role of amygdala kisspeptin in pubertal timing in female rats
| Autor: | Adekunbi, Daniel A., Li, Xiao Feng, Li, Shengyun, Adegoke, Olufeyi A., Iranloye, Bolanle O., Morakinyo, Ayodele O., Lightman, Stafford L., Taylor, Paul D., Poston, Lucilla, O’Byrne, Kevin T. |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Physiology
Maternal Health Artificial Gene Amplification and Extension Polymerase Chain Reaction Receptors G-Protein-Coupled Rats Sprague-Dawley Endocrinology Animal Cells Arcuate Nucleus Pregnancy Medicine and Health Sciences Sexual Maturation Neurons Kisspeptins Brain Obstetrics and Gynecology Amygdala Physiological Parameters Medicine Female Cellular Types Anatomy Research Article Ovulation Science Hypothalamus Research and Analysis Methods Journal Article Animals Obesity Molecular Biology Techniques Molecular Biology Nutrition Endocrine Physiology Body Weight Puberty Arcuate Nucleus of Hypothalamus Biology and Life Sciences Cell Biology Luteinizing Hormone Diet Rats Hypothalamus Anterior Cellular Neuroscience Women's Health Neuroscience Receptors Kisspeptin-1 |
| Zdroj: | PLoS ONE Adekunbi, D A, Li, X F, Li, F, Adegoke, O A, Iranloye, B O, Morakinyo, A O, Lightman, S L, Taylor, P D, Poston, L & O'Byrne, K T 2017, ' Role of amygdala kisspeptin in pubertal timing in female rats ', PL o S One, vol. 12, no. 8, e0183596 . https://doi.org/10.1371/journal.pone.0183596 PLoS ONE, Vol 12, Iss 8, p e0183596 (2017) Adekunbi, D A, Li, X F, Li, S, Adegoke, O A, Iranloye, B O, Morakinyo, A O, Lightman, S L, Taylor, P D, Poston, L & O'Byrne, K T 2017, ' Role of amygdala kisspeptin in pubertal timing in female rats ', PLoS ONE, vol. 12, no. 8, e0183596 . https://doi.org/10.1371/journal.pone.0183596 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0183596 |
| Popis: | To investigate the mechanism by which maternal obesity disrupts reproductive function in offspring, we examined Kiss1 expression in the hypothalamic arcuate (ARC) and anteroventral periventricular (AVPV) nuclei, and posterodorsal medial amygdala (MePD) of pre-pubertal and young adult offspring. Sprague-Dawley rats were fed either a standard or energy-dense diet for six weeks prior to mating and throughout pregnancy and lactation. Male and female offspring were weaned onto normal diet on postnatal day (pnd) 21. Brains were collected on pnd 30 or 100 for qRT-PCR to determine Kiss1 mRNA levels. Maternal obesity increased Kiss1 mRNA expression in the MePD of pre-pubertal male and female offspring, whereas Kiss1 expression was not affected in the ARC or AVPV at this age. Maternal obesity reduced Kiss1 expression in all three brain regions of 3 month old female offspring, but only in MePD of males. The role of MePD kisspeptin on puberty, estrous cyclicity and preovulatory LH surges was assessed directly in a separate group of post-weanling and young adult female rats exposed to a normal diet throughout their life course. Bilateral intra-MePD cannulae connected to osmotic mini-pumps for delivery of kisspeptin receptor antagonist (Peptide 234 for 14 days) were chronically implanted on pnd 21 or 100. Antagonism of MePD kisspeptin delayed puberty onset, disrupted estrous cyclicity and reduced the incidence of LH surges. These data show that the MePD plays a key role in pubertal timing and ovulation and that maternal obesity may act via amygdala kisspeptin signaling to influence reproductive function in the offspring. |
| Databáze: | OpenAIRE |
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