Prevalence and subtype distribution of Blastocystis sp. isolates from poultry in Lebanon and evidence of zoonotic potential

Autor: Greige, Stéphanie, Safadi, Dima, Bécu, Noémie, Gantois, Nausicaa, Pereira, Bruno, Chabe, Magali, Benamrouz-Vanneste, Sadia, Certad, Gabriela, Hage, Rima, Chemaly, Marianne, Hamzé, Monzer, Viscogliosi, Eric
Přispěvatelé: Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire Microbiologie Santé et Environnement [Tripoli, Liban] (LMSE), Université Libanaise, CHU Clermont-Ferrand, Faculté de gestion, économie et sciences [UCL, Lille] (FGES), Université catholique de Lille (UCL), Université catholique de Lille - Faculté de médecine et de maïeutique (UCL FMM), Groupe Hospitalier de l'Institut Catholique de Lille (GHICL), Institut de Recherche Agronomique Libanais (IRAL), Laboratoire de Ploufragan - Plouzané, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), This work was supported by grants from the Programme Orientations Stratégiques of the University of Lille 2, the Partenariat Hubert Curien (PHC) France Lebanon CEDRE 2015 Project no. 32684NM, the Centre National de la Recherche Scientifique, the Institut Pasteur of Lille, the Lebanese University and the University Catholic of Lille. SG was supported by a PhD fellowship from the AZM & Saade Association of Lebanon, The authors would like to thank all those who participated in the study, the staff of Hamidi Medical Center in Tripoli and the three poultry slaughterhouses for their assistance in sample collection and processing., Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Université Catholique de Lille - Faculté de gestion, économie et sciences (FGES), Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Université Catholique de Lille - Faculté de Médecine, Maïeutique, Sciences de la santé (FMMS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
MESH: Abattoirs
MESH: Feces/parasitology
Blastocystis Infections
Poultry
MESH: Blastocystis Infections/parasitology
Feces
Zoonosis
Subtyping
MESH: Poultry
Zoonoses
Prevalence
MESH: Blastocystis/genetics
MESH: Blastocystis/isolation & purification
MESH: Animals
MESH: Genetic Variation
Lebanon
MESH: Poultry Diseases/parasitology
MESH: Zoonoses/parasitology
MESH: Middle Aged
MESH: Real-Time Polymerase Chain Reaction
MESH: Chickens
Middle Aged
MESH: Blastocystis Infections/veterinary
MESH: Young Adult
Molecular epidemiology
MESH: Zoonoses/epidemiology
Female
MESH: Zoonoses/transmission
MESH: Lebanon/epidemiology
MESH: Poultry Diseases/transmission
Abattoirs
Adult
Avian parasitology
Intestinal parasite
Real-Time Polymerase Chain Reaction
DNA
Ribosomal
Real-time quantitative PCR
lcsh:Infectious and parasitic diseases
MESH: Blastocystis/classification
Young Adult
Animals
Humans
Transmission
lcsh:RC109-216
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Poultry Diseases
MESH: Prevalence
MESH: DNA
Ribosomal/genetics
MESH: Humans
MESH: Blastocystis Infections/transmission
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Research
Genetic Variation
Blastocystis sp
MESH: Adult
MESH: Blastocystis Infections/epidemiology
DNA
Protozoan
MESH: DNA
Protozoan/genetics
MESH: Male
Blastocystis
MESH: Poultry Diseases/epidemiology
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Chickens
MESH: Female
Zdroj: Parasites and Vectors
Parasites and Vectors, BioMed Central, 2018, 11, pp.389. ⟨10.1186/s13071-018-2975-5⟩
Parasites & Vectors, Vol 11, Iss 1, Pp 1-10 (2018)
Parasites & Vectors
Parasites & Vectors, 2018, 11, pp.389. ⟨10.1186/s13071-018-2975-5⟩
ISSN: 1756-3305
Popis: Background Blastocystis sp. is a common protozoan parasite frequently identified in the digestive tract of humans and a large variety of animal hosts worldwide, including birds. It exhibits a large genetic diversity with the identification of 17 subtypes (STs), most of them with low host specificity. ST6 and ST7 were identified in birds and suggested to represent avian STs only in the context of scarce small-scale epidemiological surveys. Moreover, these two STs also account for a significant proportion of human infections whose zoonotic origin has never been clearly confirmed. Therefore, molecular screening of Blastocystis sp. was conducted by quantitative real-time PCR for fecal samples from poultry farms and their in-contact humans from slaughterhouses in Lebanon. In parallel, a control group consisting of patients hospitalized in the same geographical area and reporting no contact with poultry was also screened for the presence of the parasite. Results The overall prevalence of Blastocystis sp. was shown to reach around 32% in chicken samples and 65% in the farms screened. All the avian isolates were subtyped and belonged to either ST6 or ST7, with a large predominance of ST6. Fifty-four percent of slaughterhouse staff members were positive for Blastocystis sp. compared with a similar prevalence of 56% in hospitalized patients. ST3 was predominant in both human cohorts followed by either ST1 then ST2 among slaughterhouse staff or by ST2 then ST1 among hospitalized patients. ST6 was also identified in two slaughterhouse workers and not in the group of hospitalized patients. Gene sequence identity was observed between chicken and human ST6 isolates from the same slaughterhouse. Conclusions Our data revealed a high prevalence of Blastocystis sp. in chicken samples and confirmed that ST6 and ST7 represented avian-adapted STs. Among both human cohorts, Blastocystis sp. infection was shown to exceed 50% with a predominance of ST3. The identification of ST6 in slaughterhouse staff members confirmed the zoonotic transmission of this ST through repeated and direct contact between chickens and their handlers. Electronic supplementary material The online version of this article (10.1186/s13071-018-2975-5) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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