| Autor: |
Inna Gladysheva, Dp, Gladyshev, Ye, Dunaevsky, Ma, Belozersky, Ni, Larionova |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Europe PubMed Central |
| ISSN: |
0006-2979 |
| Popis: |
The kinetics of binding of bovine trypsin to a proteinaceous inhibitor of trypsin from buckwheat seeds (BWI-1a) has been studied. The association rate constant (k(ass)) was 2.2 x 10(6) M-1 x sec-1 and the dissociation rate constant (k(off)) of the enzyme--inhibitor complex was 3.5 x 10(-3) sec-1; the inhibition constant Ki was 1.5 nM. The inhibitor BWI-1a is of the slow, tightly binding type. The mechanism of the inhibition of bovine trypsin by the trypsin inhibitor BWI-1a was studied. The mechanism of inhibition was found to involve two steps according to the kinetic data. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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