O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications
| Autor: | Oliver, J. A., Gómez-Millán, J., Medina, J. A., Cabeza, L., Perazzoli, G., Jimenez-Luna, C., Doello, K., Ortiz, R. |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Aged 80 and over Male rectal adenocarcinoma Rectal Neoplasms Brief Report lcsh:R lcsh:Medicine O6-methylguanine-DNA methyltransferase Chemoradiotherapy Middle Aged Immunohistochemistry Methylation Polymerase Chain Reaction O(6)-Methylguanine-DNA Methyltransferase Recurrence Humans Female Prospective Studies Aged |
| Zdroj: | Balkan Medical Journal Balkan Medical Journal, Vol 36, Iss 5, Pp 283-286 (2019) Scopus-Elsevier |
| ISSN: | 2146-3131 |
| Popis: | Aims: To analyze the clinical relevance of O6-methylguanine-DNA methyltransferase in rectal adenocarcinoma treated with chemoradiotherapy followed by surgery. Methods: Tissue samples from 29 rectal adenocarcinoma patients were obtained after chemoradiotherapy. O6-methylguanine-DNA methyltransferase promoter methylation status was established by methylation-specific polymerase chain reaction. O6-methylguanine-DNA methyltransferase protein levels were determined by immunohistochemistry. Clinicopathologic variables, including treatment regression grade, recurrence, lymph node invasion, and stage and differentiation grade of the tumor, were determined. Results: The O6-methylguanine-DNA methyltransferase gene promoter was methylated in 81.5% of samples. Most patients (88.9%) showed low O6-methylguanine-DNA methyltransferase protein expression. O6-methylguanine-DNA methyltransferase methylation status was not correlated with any of the clinicopathological variables determined in rectal adenocarcinomas selected for chemoradiotherapy. Conclusion: O6-methylguanine-DNA methyltransferase methylation status is not correlated with clinicopathologic variables examined in rectal adenocarcinoma selected for chemoradiotherapy, although its role as a biomarker awaits further investigation. |
| Databáze: | OpenAIRE |
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