Three-dimensional architecture of rat glomerular basement membrane by ultra-high resolution scanning electron microscopy

Autor:	Yamasaki, Yasushi, Makino, Hirofumi, Hironaka, Kazue, Hayashi, Yoshikazu, Shikata, Kenichi, Ota, Zensuke
Rok vydání:	1990
Předmět:	conductive staining glomerular basement membrance urogenital system Kidney Glomerulus Microscopy Electron Scanning ultra-high resolution scanning electron microscopy Animals Rats Inbred Strains ultrastructure Basement Membrane Rats
Zdroj:	Acta medica Okayama. 44(6)
ISSN:	0386-300X
Popis:	We demonstrated the ultrastructure of rat glomerular basement membrane (GBM) by ultra-high resolution scanning electron microscopy. GBM prepared by sonication methods and conductive-staining could be observed without metal coating at magnifications as high as 400,000 times. The GBM showed an irregular meshwork structure composed of various strands and pores. The width of the strands ranged from 6 to 15 nm, and the diameter of pores ranged from 6 to 50 nm. The present study confirmed our molecular sieve theory of the basement membrane.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::3e2f53bc4617aa7d38492808cc881f9c https://pubmed.ncbi.nlm.nih.gov/2075832 Zobrazit plný text záznamu