Immune responses against a new HIV-1 p24-gp41/pCAGGS-IL-12 DNA vaccine in Balb/c mice
| Autor: | Roodbari, Fatemeh, Sabahi, Farzaneh, Sarbolouki, Mohamad Nabi, Barkhordari, Farzaneh, Adeli, Ahmad, Jamedar, Amel, Mahboudi, Fereidoun |
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| Přispěvatelé: | Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University [Tehran], Department of Cellular and Molecular Biology, Faculty of Basic Sciences, University of Mazandaran, Institute of Biochemistry and Biophysics, Tehran University of Basic Sciences, Biotechnology Research Center, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Department of Microbiology and Virology, Faculty of Medicine, Mashad University of Medical Sciences, This work was supported by a grant from Pasteur Institute of Iran, The authors would like to acknowledge the contributions of Ms. Rozita Edalat and Dr. Abbas Jamali in this research. |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Interleukin-12/metabolism
viruses HIV Core Protein p24 HIV Infections MESH: AIDS Vaccines/immunology MESH: Recombinant Fusion Proteins/genetics Mice Vaccines DNA DNA Vaccine Dendrosome MESH: Animals MESH: Interferon-gamma/metabolism MESH: Immunity Humoral MESH: Interleukin-4/blood Cells Cultured AIDS Vaccines Immunity Cellular Mice Inbred BALB C virus diseases MESH: HIV Core Protein p24/immunology p24 gp41 Interleukin-12 HIV Envelope Protein gp41 MESH: HIV Envelope Protein gp41/genetics MESH: HIV Infections/prevention & control MESH: HIV Core Protein p24/genetics MESH: Recombinant Fusion Proteins/immunology IL-12 MESH: Immunization [SDV.IMM]Life Sciences [q-bio]/Immunology Female MESH: HIV-1/immunology MESH: Vaccines DNA/immunology MESH: Cells Cultured MESH: HIV Envelope Protein gp41/immunology Recombinant Fusion Proteins MESH: Mice Inbred BALB C MESH: Immunity Cellular Interferon-gamma MESH: Interleukin-12/genetics Animals Humans MESH: Mice MESH: Humans Immunity Humoral Immunoglobulin G HIV-1 Immunization Interleukin-4 MESH: Immunoglobulin G/blood MESH: Female |
| Zdroj: | Iranian Journal of Immunology Iranian Journal of Immunology, Shiraz Institute for Cancer Research, 2012, 9 (2), pp.86-97 |
| ISSN: | 1735-367X 1735-1383 |
| Popis: | International audience; Background: Development of an effective vaccine is highly needed in order to restrict the AIDS pandemic. DNA vaccines initiate both arms of immunity without the potential of causing disease. HIV-1 p24 and gp41 (gag and env) proteins play important roles in viral pathogenesis and are effective candidates for immune induction and vaccine design. Objective: In this study, new DNA vaccine candidates constructed from HIV-1 fused p24-gp41 or gp41 alone were evaluated in Balb/c mice for induction of cellular and humoral immune responses. Methods: Recombinant plasmids, pcDNA3.1/Hygro expression vector containing immunogenic sequences of fused p24-gp41 or gp41alone were produced. Dendrosome used as a system for carrying vectors in laboratory animals, and an IL-12 containing vector (pCAGGS-IL-12) was co-immunized with the p24-gp41 vector as a genetic adjuvant. Induction of effective immune responses against the designed vectors as DNA vaccine candidates in Balb/c mice was evaluated. Levels of total antibodies, IgG isotypes (IgG2a and IgG1); IFN-γ and IL-4 were measured by ELISA. MTT assay was used to evaluate lymphoproliferation. Results: The results confirmed that the immunogenic epitopes of both p24 and gp41 genes are highly effective inducers of immune responses, and administration of fused p24-gp41 alone or along with IL-12 resulted in further enhancement of immune responses. Group 4 that received fused fragments (p24-gp41) along with an IL-12 expressing vector demonstrated a significantly higher Stimulation Index (SI) and IFN-γ production (p |
| Databáze: | OpenAIRE |
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