Intramyocardial protein therapy with vascular endothelial growth factor (VEGF-165) induces functional angiogenesis in rat senescent myocardium
| Autor: | Besse, Sophie, Boucher, François, Linguet, Gilberte, Riou, Laurent, De Leiris, Joël, Riou, Bertrand, Dimastromatteo, Julien, Comte, René, Courty, José, Delbe, Jean |
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| Přispěvatelé: | Croissance cellulaire, réparation et régénération tissulaires (CRRET), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée (TIMC-IMAG-PRETA), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Radiopharmaceutiques biocliniques (LRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF) |
| Rok vydání: | 2010 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A MESH: Myocardium MESH: Rats Down-Regulation Neovascularization Physiologic MESH: Down-Regulation MESH: Recombinant Proteins [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] MESH: Coronary Vessels Animals Humans MESH: Animals Rats Wistar MESH: Age Factors Heart Failure MESH: Humans MESH: Vascular Endothelial Growth Factor Receptor-2 MESH: Vascular Endothelial Growth Factor A Myocardium Age Factors MESH: Rats Wistar Coronary Vessels Vascular Endothelial Growth Factor Receptor-2 MESH: Male Recombinant Proteins Rats MESH: Heart Failure Hypertrophy Left Ventricular MESH: Hypertrophy Left Ventricular MESH: Neovascularization Physiologic |
| Zdroj: | Journal of Physiology and Pharmacology Journal of Physiology and Pharmacology, Krakow Polish Physiological Society, 2010, 61 (6), pp.651-61 Europe PubMed Central ResearcherID |
| ISSN: | 1899-1505 0867-5910 |
| Popis: | International audience; Myocardial capillary density and angiogenesis are impaired during aging but whether growth factor therapy is able to induce functional neovascularization in senescent heart have never been studied. In 3, 24, 28 and 32 mo male Wistar rats, cardiac hemodynamic measurements indicated heart failure at 28 and 32 mo, associated with left ventricular hypertrophy. VEGF/VEGF-R2, Ang-1/Ang-2/Tie-2 and PTN levels, quantitated in left ventricle by western blotting and immunohistochemistry, showed that VEGF and VEGF-R2 levels were specifically decreased during aging. In vitro angiogenesis ± rhVEGF-165 (5 and 50 ng/mL) was measured in aortic segments in 3D-collagen. Aortic sprouting was decreased during aging but restored by VEGF treatment (P |
| Databáze: | OpenAIRE |
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