Differential Susceptibility and Innate Immune Response of Aedes aegypti and Aedes albopictus to the Haitian Strain of the Mayaro Virus
| Autor: | Diop, Fode, Alout, Haoues, Diagne, Cheikh, Bengue, Michèle, Baronti, Cecile, Hamel, Rodolphe, Talignani, Loïc, Liegeois, Florian, Pompon, Julien, Morales Vargas, Ronald, Nougairède, Antoine, Missé, Dorothée |
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| Přispěvatelé: | Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Interhuman Arbovirus Transmission (MIVEGEC-iHAT), Biologie des infections virales: Emergence, DIFfusion, Impact, Contrôle, Elimination (EDIFICE), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Virostyle (MIVEGEC-Virostyle), Perturbations, Evolution, Virulence (PEV), Zoonoses virales et MTN (MIVEGEC-VIROZ), Mahidol University [Bangkok], ANR-16-IDEX-0006,MUSE,MUSE(2016), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Herrada, Anthony, Génétique et évolution des maladies infectieuses (GEMI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Génétique et évolution des maladies infectieuses (GEMI) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
[SDV.IMM] Life Sciences [q-bio]/Immunology
education lcsh:QR1-502 chondrocytes Gene Expression Alphavirus Mosquito Vectors Real-Time Polymerase Chain Reaction niemann–pick type c1 Article lcsh:Microbiology Aedes aegypti Aedes [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Animals ML26A Niemann-Pick type C1 Alphavirus Infections aedes aegypti Gene Expression Profiling fungi virus diseases Aedes albopictus Mayaro virus Immunity Innate arbovirus aedes albopictus [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.IMM]Life Sciences [q-bio]/Immunology Serine Proteases Antimicrobial Cationic Peptides |
| Zdroj: | Viruses Viruses, 2019, 11 (10), pp.924. ⟨10.3390/v11100924⟩ Viruses, MDPI, 2019, 11 (10), pp.924. ⟨10.3390/v11100924⟩ Viruses, Vol 11, Iss 10, p 924 (2019) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v11100924⟩ |
| Popis: | Mayaro (MAYV) is an emerging arthropod-borne virus belonging to the Alphavirus genus of the Togaviridae family. Although forest-dwelling Haemagogus mosquitoes have been considered as its main vector, the virus has also been detected in circulating Aedes ssp mosquitoes. Here we assess the susceptibility of Aedes aegypti and Aedes albopictus to infection with MAYV and their innate immune response at an early stage of infection. Aedes albopictus was more susceptible to infection with MAYV than Ae. aegypti. Analysis of transcript levels of twenty immunity-related genes by real-time PCR in the midgut of both mosquitoes infected with MAYV revealed increased expression of several immune genes, including CLIP-domain serine proteases, the anti-microbial peptides defensin A, E, cecropin E, and the virus inducible gene. The regulation of certain genes appeared to be Aedes species-dependent. Infection of Ae. aegypti with MAYV resulted in increased levels of myeloid differentiation2-related lipid recognition protein (ML26A) transcripts, as compared to Ae. albopictus. Increased expression levels of thio-ester-containing protein 22 (TEP22) and Niemann−Pick type C1 (NPC1) gene transcripts were observed in infected Ae. albopictus, but not Ae. aegypti. The differences in these gene expression levels during MAYV infection could explain the variation in susceptibility observed in both mosquito species. |
| Databáze: | OpenAIRE |
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