Detection of hyperreactive T cells in multiple myeloma by multivalent cross-linking of the CD3/TCR complex
| Autor: | Massimo MASSAIA, Bianchi, A., Attisano, C., Peola, S., Redoglia, V., Dianzani, U., Pileri, A. |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Immunity Cellular CD3 Complex CD8 Antigens Ionomycin T-Lymphocytes Receptors Antigen T-Cell Antibodies Monoclonal Receptors Interleukin-2 HLA-DR Antigens T-Lymphocytes Helper-Inducer T-Lymphocytes Regulatory Immunophenotyping Leukocyte Count Antigens CD Histocompatibility Antigens Humans Interleukin-2 Leukocyte Common Antigens Tetradecanoylphorbol Acetate Multiple Myeloma Aged |
| Zdroj: | Europe PubMed Central Scopus-Elsevier |
| Popis: | Cellular immunity was investigated in 43 patients with multiple myeloma (MM) by assessing 3HTdR uptake induced by monocyte-dependent [CD3 monoclonal antibodies (MoAbs), phytohemagglutinin (PHA)] and monocyte-independent (CD2 MoAbs, ionomycin + phorbolester) stimulations. The former were evaluated in peripheral blood mononuclear cells (PBMNC) and purified T cells; the latter were evaluated in purified T-cell preparations only. MM showed significantly lower PBMNC responses to PHA (P less than .001), soluble OKT3 (CD3) (P = .01), and immobilized OKT3 MoAbs (P = .01). On purification of T cells, MM responses were still defective to soluble T11(2) + T11(3) (CD2) MoAbs (P = .004), phorbol myristate acetate (PMA) plus ionomycin (P less than .001), but significantly higher to plastic-immobilized OKT3 (P = .004). In some MM, 3HTdR uptake, interleukin-2 (IL-2) receptor (CD25) expression, and IL-2 production were as high on stimulation with plastic-immobilized OKT3 as that observed in normal subjects under optimal conditions (ie, plastic-immobilized OKT3 plus accessory signals). CD3 hyperreactivity correlated with the number of CD8+ HLA-DR+ cells in MM T-cell preparations. MM patients with more than 10% CD8+ HLA-DR+ cells had significantly higher responses to immobilized OKT3 (P less than .001), but lower responses to T11(2) plus T11(3) (P = .01), and PMA plus ionomycin (P = .03) than patients with less than 10% CD8+ HLA-DR+ cells. Phenotyping of CD45RA (naive) and CD45R0 (memory) expressions in resting MM T cells showed a lower ratio of CD45RA to CD45R0 in both CD4 (P less than .05) and CD8 (P less than .001) subpopulations. These data indicate that (a) some MM T cells require significantly fewer accessory signals (if any) to express the IL-2 receptor fully, secrete IL-2, and proliferate on multivalent cross-linking of the CD3/TCR complex; and (b) this peculiar state of activation is associated with high HLA-DR expression in CD8+ lymphocytes. |
| Databáze: | OpenAIRE |
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