Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway
| Autor: | Barreca, Maria Magdalena, Walter Spinello, Cavalieri, Vincenzo, Turturici, G., Sconzo, G., Kaur, P., Tinnirello, R., Asea, A., Fabiana Geraci |
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| Přispěvatelé: | Barreca, M., Spinello, W., Cavalieri, V., Turturici, G., Sconzo, G., Kaur, P., Tinnirello, R., Asea, A., Geraci, F. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Extracellular Vesicle
NF-kappa B Endothelial Cells Models Biological Hsp70 Toll-Like Receptor 4 Autocrine Communication Mice Phosphatidylinositol 3-Kinases Membrane Microdomains Matrix Metalloproteinase 9 NF-KappaB Inhibitor alpha Cell Movement Mesoangioblast Stem Cell Animals Matrix Metalloproteinase 2 HSP70 Heat-Shock Proteins Extracellular Space Matrix Metalloproteinase Proto-Oncogene Proteins c-akt Low Density Lipoprotein Receptor-Related Protein-1 Migration Protein Binding Signal Transduction |
| Zdroj: | Università degli Studi di Palermo-IRIS |
| ISSN: | 1845-1861 |
| Popis: | Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor 4 (TLR4) and CD91 to stimulate migration. We further demonstrate that Hsp70 has a positive role in regulating matrix metalloproteinase 2 (MMP2) and MMP9 expression and that MMP2 has a more pronounced effect on cell migration, as compared to MMP9. In addition, the analysis of the intracellular pathways implicated in Hsp70 regulated signal transduction showed the involvement of both PI3K/AKT and NF-κB. Taken together, our findings present a paradigm shift in our understanding of the molecular mechanisms that regulate mesoangioblast stem cells ability to traverse the extracellular matrix (ECM). J. Cell. Physiol. 232: 1845-1861, 2017. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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