A fine-tuned β-catenin regulation during proliferation of corneal endothelial cells revealed using proteomics analysis

Autor:	Maurizi, E., Schiroli, D., Zini, R., Limongelli, A., Misto, R., Macaluso, C., Pellegrini, G.
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Proteomics Cell biology Epithelial-Mesenchymal Transition Molecular biology Endothelium Corneal lcsh:R Endothelial Cells lcsh:Medicine Resting Phase Cell Cycle Biochemistry Article Transforming Growth Factor beta Animals lcsh:Q Rabbits lcsh:Science Cells Cultured beta Catenin Cell Proliferation Signal Transduction
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) Scientific Reports
ISSN:	2045-2322
DOI:	10.1038/s41598-020-70800-w
Popis:	Corneal endothelial (CE) dysfunction is the main indication for corneal transplantation, an invasive procedure with several limitations. Developing novel strategies to re-activate CE regenerative capacity is, therefore, of fundamental importance. This goal has proved to be challenging as corneal endothelial cells (CEnC) are blocked in the G0/G1 phase of the cell cycle in vivo and, albeit retaining proliferative capacity in vitro, this is further hindered by endothelial-to-mesenchymal transition. Herein we investigated the mechanisms regulating CEnC proliferation in vitro. Comparing the proteome of non-proliferating (in vivo—G0/G1) and proliferating (in vitro—G2/M) rabbit CEnC (rCEnC), 77 proteins, out of 3,328 identified, were differentially expressed in the two groups (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::36a71efe773ab18208d4008ae7c84ac2 http://link.springer.com/article/10.1038/s41598-020-70800-w Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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